TY - JOUR
T1 - Negative regulation of immediate-early gene expression of pseudorabies virus by interferon-
AU - Tonomura, Noriko
AU - Ono, Etsuro
AU - Shimizu, Yukio
AU - Kida, Hiroshi
N1 - Funding Information:
This work was supported by Grants-in-Aid for Scientific Research No. 05506002 from the Ministry of Education, Science, Sports and Culture, Japan. We thank Hayashibara Biochemical Laboratories, Inc. for providing natural human interferon-a and monoclonal antibody to the interferon-a, and Dr. S. Yamada for the gift of plasmid pG3S.
PY - 1996/12
Y1 - 1996/12
N2 - Pseudorabies virus (PrV) replication in Vero cells was suppressed by treatment with human natural interferon-α (IFN-α), Messenger RNA transcribed from the PrV immediate-early (IE) gene was reduced in the IFN-α-treated cells. Transient expression assays showed that transcription from the PrV IE promoter was selectively inhibited in the IFN-α-treated cells. Analysis of deletion mutants of the PrV IE promoter sequence suggested that at least one element between the transcription initiation site (+ 1) and -90 in the PrV IE promoter was concerned with the negative regulation.
AB - Pseudorabies virus (PrV) replication in Vero cells was suppressed by treatment with human natural interferon-α (IFN-α), Messenger RNA transcribed from the PrV immediate-early (IE) gene was reduced in the IFN-α-treated cells. Transient expression assays showed that transcription from the PrV IE promoter was selectively inhibited in the IFN-α-treated cells. Analysis of deletion mutants of the PrV IE promoter sequence suggested that at least one element between the transcription initiation site (+ 1) and -90 in the PrV IE promoter was concerned with the negative regulation.
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U2 - 10.1016/S0378-1135(96)01260-6
DO - 10.1016/S0378-1135(96)01260-6
M3 - Article
C2 - 9008338
AN - SCOPUS:0030443113
SN - 0378-1135
VL - 53
SP - 271
EP - 281
JO - Veterinary Microbiology
JF - Veterinary Microbiology
IS - 3-4
ER -