TY - JOUR
T1 - Needle-free immunization using a solid-in-oil nanodispersion enhanced by a skin-permeable oligoarginine peptide
AU - Kitaoka, Momoko
AU - Imamura, Kana
AU - Hirakawa, Yuya
AU - Tahara, Yoshiro
AU - Kamiya, Noriho
AU - Goto, Masahiro
N1 - Funding Information:
This work was financed by a Grant-in-Aid for Scientific Research (S) 24226019 from the Ministry of Education, Culture, Sports, Science, and Technology of Japan (to M.G. and N.K.). We thank Professors Takuro Niidome and Yoshiki Katayama for their assistance with the animal experiments.
PY - 2013
Y1 - 2013
N2 - The objective of transcutaneous immunization is efficient vaccination using the skin's immune system. Although a less invasive administration procedure is involved, the effective delivery of antigen using this modality remains a problem. Here, we demonstrate the use of a solid-in-oil (S/O) nanodispersion system for the transcutaneous immunization of male ddY mice with ovalbumin (OVA) antigen. The S/O nanoparticles consisted of OVA and hydrophobic surfactant molecules and were dispersed in oil; enhanced induction of antigen-specific antibody was observed after the addition of polyarginine (R6) into the same S/O nanoparticle containing OVA. The improved S/O nanodispersion system induced a comparable level of OVA-specific antibody to that induced by subcutaneous injection of OVA at the same dose, advocating the potential application of the S/O system as a needle-free and easy-to-use immunization system.
AB - The objective of transcutaneous immunization is efficient vaccination using the skin's immune system. Although a less invasive administration procedure is involved, the effective delivery of antigen using this modality remains a problem. Here, we demonstrate the use of a solid-in-oil (S/O) nanodispersion system for the transcutaneous immunization of male ddY mice with ovalbumin (OVA) antigen. The S/O nanoparticles consisted of OVA and hydrophobic surfactant molecules and were dispersed in oil; enhanced induction of antigen-specific antibody was observed after the addition of polyarginine (R6) into the same S/O nanoparticle containing OVA. The improved S/O nanodispersion system induced a comparable level of OVA-specific antibody to that induced by subcutaneous injection of OVA at the same dose, advocating the potential application of the S/O system as a needle-free and easy-to-use immunization system.
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U2 - 10.1016/j.ijpharm.2013.10.006
DO - 10.1016/j.ijpharm.2013.10.006
M3 - Article
C2 - 24409521
AN - SCOPUS:84889096988
SN - 0378-5173
VL - 458
SP - 334
EP - 339
JO - International Journal of Pharmaceutics
JF - International Journal of Pharmaceutics
IS - 2
ER -
