Near Infrared Photoimmunotherapy with Combined Exposure of External and Interstitial Light Sources

Yasuhiro Maruoka, Tadanobu Nagaya, Kazuhide Sato, Fusa Ogata, Shuhei Okuyama, Peter L. Choyke, Hisataka Kobayashi

Research output: Contribution to journalArticlepeer-review

42 Citations (Scopus)

Abstract

Near infrared photoimmunotherapy (NIR-PIT) is a new target-cell-specific cancer treatment that induces highly selective necrotic/immunogenic cell death after systemic administration of a photoabsorber antibody conjugate and subsequent NIR light exposure. However, the depth of NIR light penetration in tissue (approximately 2 cm) with external light sources limits the therapeutic effects of NIR-PIT. Interstitial light exposure using cylindrical diffusing optical fibers can overcome this limitation. The purpose in this study was to compare three NIR light delivery methods for treating tumors with NIR-PIT using a NIR laser system at an identical light energy; external exposure alone, interstitial exposure alone, and the combination. Panitumumab conjugated with the photoabsorber IRDye-700DX (pan-IR700) was intravenously administered to mice with A431-luc xenografts which are epithelial growth factor receptor (EGFR) positive. One and 2 days later, NIR light was administered to the tumors using one of three methods. Interstitial exposure alone and in combination with external sources showed the greatest decrease in bioluminescence signal intensity. Additionally, the combination of external and interstitial NIR light exposure showed significantly greater tumor size reduction and prolonged survival after NIR-PIT compared to external exposure alone. This result suggested that the combination of external and interstitial NIR light exposure was more effective than externally applied light alone. Although external exposure is the least invasive means of delivering light, the combination of external and interstitial exposures produces superior therapeutic efficacy in tumors greater than 2 cm in depth from the tissue surface.

Original languageEnglish
Pages (from-to)3634-3641
Number of pages8
JournalMolecular pharmaceutics
Volume15
Issue number9
DOIs
Publication statusPublished - Sept 4 2018
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Molecular Medicine
  • Pharmaceutical Science
  • Drug Discovery

Fingerprint

Dive into the research topics of 'Near Infrared Photoimmunotherapy with Combined Exposure of External and Interstitial Light Sources'. Together they form a unique fingerprint.

Cite this