Nanopillar chips arranged in tilted array pattern for fast separation of DNA and proteins

Takao Yasui; Noritada Kaji; Mohamad Reza Mohamadi; Ryo Ogawa; Shingi Hashioka; Manabu Tokeshi; Yasuhiro Horiike; Yoshinobu Baba

Nanopillar chips arranged in tilted array pattern for fast separation of DNA and proteins

Takao Yasui, Noritada Kaji, Mohamad Reza Mohamadi, Ryo Ogawa, Shingi Hashioka, Manabu Tokeshi, Yasuhiro Horiike, Yoshinobu Baba

Research output: Contribution to conference › Paper › peer-review

3 Citations (Scopus)

Abstract

In this paper, EOF mesurement and biomolecular separation (21-340 kDa in proteins, 1-48.5 kbp in DNA) were carefully studied by using nanopillar chips arranged in tilted array pattern with 100-nm spacing. We revealed that the nanopillar chips had an intrinstic function to suppress electro-osmotic flow (EOF), and as a result, DNA and SDS-protein complexes could be separated without any sieving matrices and surface coatings.

Original language	English
Pages	432-434
Number of pages	3
Publication status	Published - 2008
Externally published	Yes
Event	12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008 - San Diego, CA, United States Duration: Oct 12 2008 → Oct 16 2008

Other

Other	12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008
Country/Territory	United States
City	San Diego, CA
Period	10/12/08 → 10/16/08

All Science Journal Classification (ASJC) codes

Chemical Engineering (miscellaneous)
Bioengineering

Cite this

Nanopillar chips arranged in tilted array pattern for fast separation of DNA and proteins. / Yasui, Takao; Kaji, Noritada; Mohamadi, Mohamad Reza et al.
2008. 432-434 Paper presented at 12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008, San Diego, CA, United States.

Research output: Contribution to conference › Paper › peer-review

Yasui, T, Kaji, N, Mohamadi, MR, Ogawa, R, Hashioka, S, Tokeshi, M, Horiike, Y & Baba, Y 2008, 'Nanopillar chips arranged in tilted array pattern for fast separation of DNA and proteins', Paper presented at 12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008, San Diego, CA, United States, 10/12/08 - 10/16/08 pp. 432-434.

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AU - Yasui, Takao

AU - Kaji, Noritada

AU - Mohamadi, Mohamad Reza

AU - Ogawa, Ryo

AU - Hashioka, Shingi

AU - Tokeshi, Manabu

AU - Horiike, Yasuhiro

AU - Baba, Yoshinobu

PY - 2008

Y1 - 2008

N2 - In this paper, EOF mesurement and biomolecular separation (21-340 kDa in proteins, 1-48.5 kbp in DNA) were carefully studied by using nanopillar chips arranged in tilted array pattern with 100-nm spacing. We revealed that the nanopillar chips had an intrinstic function to suppress electro-osmotic flow (EOF), and as a result, DNA and SDS-protein complexes could be separated without any sieving matrices and surface coatings.

AB - In this paper, EOF mesurement and biomolecular separation (21-340 kDa in proteins, 1-48.5 kbp in DNA) were carefully studied by using nanopillar chips arranged in tilted array pattern with 100-nm spacing. We revealed that the nanopillar chips had an intrinstic function to suppress electro-osmotic flow (EOF), and as a result, DNA and SDS-protein complexes could be separated without any sieving matrices and surface coatings.

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T2 - 12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008

Y2 - 12 October 2008 through 16 October 2008

ER -

Nanopillar chips arranged in tilted array pattern for fast separation of DNA and proteins

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