N-cadherin is regulated by activin A and associated with tumor aggressiveness in esophageal carcinoma

Keiji Yoshinaga, Hiroshi Inoue, Tohru Utsunomiya, Hideto Sonoda, Takaaki Masuda, Koshi Mimori, Yoichi Tanaka, Masaki Mori

Research output: Contribution to journalArticlepeer-review

47 Citations (Scopus)


Purpose: Activin A is a member of the transforming growth factor β superfamily and plays an important role in the differentiation of embryonic stem cells. We have reported previously that the expression of activin A is associated with lymph node metastasis in esophageal cancer, and our purpose in the current work is to clarify the molecular mechanism of the aggressive behavior of tumors that have high activin A expression. Experimental Design: We have compared the gene expression profiles of human esophageal carcinoma cell lines that were stably transfected with activin βA, which is a subunit of activin A, with those of control human esophageal carcinoma cell lines, using a cDNA microarray. Results: We found that the expression level of neuronal cadherin (N-cadherin) was higher in the transfectants than in the control cells. N-cadherin was located on the cell surface of the transfectants, irrespective of the expression of epithelial cadherin (E-cadherin), and the expression of N-cadherin mRNA was significantly associated with that of activin βA mRNA in clinical samples of esophageal carcinoma (n = 51; r = 0.855). A clinicopathologic analysis suggested that expression of N-cadherin mRNA was associated with the depth of tumor wall invasion, and a group of patients with high expression of N-cadherin mRNA showed a significantly poorer prognosis than a group of patients with low N-cadherin expression (P = 0.046). Conclusions: These results indicate that activin A might mediate the expression of N-cadherin and that this may be associated with depth of invasion and poor prognosis.

Original languageEnglish
Pages (from-to)5702-5707
Number of pages6
JournalClinical Cancer Research
Issue number17
Publication statusPublished - Sept 1 2004

All Science Journal Classification (ASJC) codes

  • Oncology
  • Cancer Research


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