TY - JOUR
T1 - Myostatin alters glucose transporter-4 (GLUT4) expression in bovine skeletal muscles and myoblasts isolated from double-muscled (DM) andnormal-muscled (NM) Japanese shorthorn cattle
AU - Takahashi, H.
AU - Sato, K.
AU - Yamaguchi, T.
AU - Miyake, M.
AU - Watanabe, H.
AU - Nagasawa, Y.
AU - Kitagawa, E.
AU - Terada, S.
AU - Urakawa, M.
AU - Rose, M. T.
AU - McMahon, C. D.
AU - Watanabe, K.
AU - Ohwada, S.
AU - Gotoh, T.
AU - Aso, H.
N1 - Funding Information:
We are grateful to Noriya Oosaki, Toshiyuki Hirata, Mituyoshi Satoh, Tomio Sato for their excellent help in conducting this study. This study was supported by a grant-in-aid for the Program for Promotion of Basic and Applied Research for Innovations in Bio-oriented Industry and by JSPS KAKENHI grant number 24780266 .
PY - 2014/7
Y1 - 2014/7
N2 - The purpose of this study was to determine whether myostatin alters glucose transporter-4 (GLUT4) expression in bovine skeletal muscles and myoblasts isolated from double-muscled (DM) and normal-muscled (NM) Japanese Shorthorn cattle. Plasma concentrations of glucose were lower in DM cattle than in NM cattle (P < 0.01). The expression of GLUT4 messenger RNA (mRNA) in the skeletal muscle exvivo and in myoblasts at 72h after differentiation invitro was higher in DM cattle than in NM cattle (P < 0.01). In contrast, the NM and DM cattle did not differ with respect to skeletal muscle expression of GLUT1 and myocyte enhancer factor-2c (MEF2c), a transcription factor of GLUT4. In differentiated myoblasts, the expression of GLUT1, GLUT4, and MEF2c mRNAs was greater in DM cattle than in NM cattle (P < 0.01). In the presence and absence of insulin, glucose uptake in myoblasts was increased in DM cattle relative to that of NM cattle (P < 0.01). The addition of myostatin decreased the expression of GLUT4 and MEF2c mRNAs in DM myoblasts (P < 0.05). Results of the present study suggest that myostatin inhibits the expression of GLUT4 mRNA possibly via MEF2c and that the greater ability of the DM cattle to produce muscle relative to the NM cattle may be due to their greater sensitivity to insulin and greater use of glucose.
AB - The purpose of this study was to determine whether myostatin alters glucose transporter-4 (GLUT4) expression in bovine skeletal muscles and myoblasts isolated from double-muscled (DM) and normal-muscled (NM) Japanese Shorthorn cattle. Plasma concentrations of glucose were lower in DM cattle than in NM cattle (P < 0.01). The expression of GLUT4 messenger RNA (mRNA) in the skeletal muscle exvivo and in myoblasts at 72h after differentiation invitro was higher in DM cattle than in NM cattle (P < 0.01). In contrast, the NM and DM cattle did not differ with respect to skeletal muscle expression of GLUT1 and myocyte enhancer factor-2c (MEF2c), a transcription factor of GLUT4. In differentiated myoblasts, the expression of GLUT1, GLUT4, and MEF2c mRNAs was greater in DM cattle than in NM cattle (P < 0.01). In the presence and absence of insulin, glucose uptake in myoblasts was increased in DM cattle relative to that of NM cattle (P < 0.01). The addition of myostatin decreased the expression of GLUT4 and MEF2c mRNAs in DM myoblasts (P < 0.05). Results of the present study suggest that myostatin inhibits the expression of GLUT4 mRNA possibly via MEF2c and that the greater ability of the DM cattle to produce muscle relative to the NM cattle may be due to their greater sensitivity to insulin and greater use of glucose.
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U2 - 10.1016/j.domaniend.2014.01.007
DO - 10.1016/j.domaniend.2014.01.007
M3 - Article
C2 - 24906930
AN - SCOPUS:84896512497
SN - 0739-7240
VL - 48
SP - 62
EP - 68
JO - Domestic Animal Endocrinology
JF - Domestic Animal Endocrinology
IS - 1
ER -