Myogenic microRNA expression requires ATP-dependent chromatin remodeling enzyme function

Chandrashekara Mallappa; Brian T. Nasipak; Letitiah Etheridge; Elliot J. Androphy; Stephen N. Jones; Charles G. Sagerström; Yasuyuki Ohkawa; Anthony N. Imbalzano

doi:10.1128/MCB.00214-10

Myogenic microRNA expression requires ATP-dependent chromatin remodeling enzyme function

Chandrashekara Mallappa, Brian T. Nasipak, Letitiah Etheridge, Elliot J. Androphy, Stephen N. Jones, Charles G. Sagerström, Yasuyuki Ohkawa, Anthony N. Imbalzano

Research output: Contribution to journal › Article › peer-review

26 Citations (Scopus)

Abstract

Knockdown of the Brg1 ATPase subunit of SWI/SNF chromatin remodeling enzymes in developing zebrafish caused stunted tail formation and altered sarcomeric actin organization, which phenocopies the loss of the microRNA processing enzyme Dicer, or the knockdown of myogenic microRNAs. Furthermore, myogenic microRNA expression and differentiation was blocked in Brg1 conditional myoblasts differentiated ex vivo. The binding of Brg1 upstream of myogenic microRNA sequences correlated with MyoD binding and accessible chromatin structure in satellite cells and myofibers, and it was required for chromatin accessibility and microRNA expression in a tissue culture model for myogenesis. The results implicate ATP-dependent chromatin remodelers in myogenic microRNA gene regulation.

Original language	English
Pages (from-to)	3176-3186
Number of pages	11
Journal	Molecular and cellular biology
Volume	30
Issue number	13
DOIs	https://doi.org/10.1128/MCB.00214-10
Publication status	Published - Jul 2010

All Science Journal Classification (ASJC) codes

Molecular Biology
Cell Biology

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title = "Myogenic microRNA expression requires ATP-dependent chromatin remodeling enzyme function",

abstract = "Knockdown of the Brg1 ATPase subunit of SWI/SNF chromatin remodeling enzymes in developing zebrafish caused stunted tail formation and altered sarcomeric actin organization, which phenocopies the loss of the microRNA processing enzyme Dicer, or the knockdown of myogenic microRNAs. Furthermore, myogenic microRNA expression and differentiation was blocked in Brg1 conditional myoblasts differentiated ex vivo. The binding of Brg1 upstream of myogenic microRNA sequences correlated with MyoD binding and accessible chromatin structure in satellite cells and myofibers, and it was required for chromatin accessibility and microRNA expression in a tissue culture model for myogenesis. The results implicate ATP-dependent chromatin remodelers in myogenic microRNA gene regulation.",

author = "Chandrashekara Mallappa and Nasipak, {Brian T.} and Letitiah Etheridge and Androphy, {Elliot J.} and Jones, {Stephen N.} and Sagerstr{\"o}m, {Charles G.} and Yasuyuki Ohkawa and Imbalzano, {Anthony N.}",

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T1 - Myogenic microRNA expression requires ATP-dependent chromatin remodeling enzyme function

AU - Mallappa, Chandrashekara

AU - Nasipak, Brian T.

AU - Etheridge, Letitiah

AU - Androphy, Elliot J.

AU - Jones, Stephen N.

AU - Sagerström, Charles G.

AU - Ohkawa, Yasuyuki

AU - Imbalzano, Anthony N.

PY - 2010/7

Y1 - 2010/7

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AB - Knockdown of the Brg1 ATPase subunit of SWI/SNF chromatin remodeling enzymes in developing zebrafish caused stunted tail formation and altered sarcomeric actin organization, which phenocopies the loss of the microRNA processing enzyme Dicer, or the knockdown of myogenic microRNAs. Furthermore, myogenic microRNA expression and differentiation was blocked in Brg1 conditional myoblasts differentiated ex vivo. The binding of Brg1 upstream of myogenic microRNA sequences correlated with MyoD binding and accessible chromatin structure in satellite cells and myofibers, and it was required for chromatin accessibility and microRNA expression in a tissue culture model for myogenesis. The results implicate ATP-dependent chromatin remodelers in myogenic microRNA gene regulation.

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Myogenic microRNA expression requires ATP-dependent chromatin remodeling enzyme function

Abstract

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