Myogenic microRNA expression requires ATP-dependent chromatin remodeling enzyme function

Chandrashekara Mallappa, Brian T. Nasipak, Letitiah Etheridge, Elliot J. Androphy, Stephen N. Jones, Charles G. Sagerström, Yasuyuki Ohkawa, Anthony N. Imbalzano

    Research output: Contribution to journalArticlepeer-review

    26 Citations (Scopus)


    Knockdown of the Brg1 ATPase subunit of SWI/SNF chromatin remodeling enzymes in developing zebrafish caused stunted tail formation and altered sarcomeric actin organization, which phenocopies the loss of the microRNA processing enzyme Dicer, or the knockdown of myogenic microRNAs. Furthermore, myogenic microRNA expression and differentiation was blocked in Brg1 conditional myoblasts differentiated ex vivo. The binding of Brg1 upstream of myogenic microRNA sequences correlated with MyoD binding and accessible chromatin structure in satellite cells and myofibers, and it was required for chromatin accessibility and microRNA expression in a tissue culture model for myogenesis. The results implicate ATP-dependent chromatin remodelers in myogenic microRNA gene regulation.

    Original languageEnglish
    Pages (from-to)3176-3186
    Number of pages11
    JournalMolecular and cellular biology
    Issue number13
    Publication statusPublished - Jul 2010

    All Science Journal Classification (ASJC) codes

    • Molecular Biology
    • Cell Biology


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