@article{3aa3905faea4434e9488b8ef23caf5e1,
title = "MYB transcription factor gene involved in sex determination in Asparagus officinalis",
abstract = "Dioecy is a plant mating system in which individuals of a species are either male or female. Although many flowering plants evolved independently from hermaphroditism to dioecy, the molecular mechanism underlying this transition remains largely unknown. Sex determination in the dioecious plant Asparagus officinalis is controlled by X and Y chromosomes; the male and female karyotypes are XY and XX, respectively. Transcriptome analysis of A. officinalis buds showed that a MYB-like gene, Male Specific Expression 1 (MSE1), is specifically expressed in males. MSE1 exhibits tight linkage with the Y chromosome, specific expression in early anther development and loss of function on the X chromosome. Knockout of the MSE1 orthologue in Arabidopsis induces male sterility. Thus, MSE1 acts in sex determination in A. officinalis.",
author = "Kohji Murase and Shuji Shigenobu and Sota Fujii and Kazuki Ueda and Takanori Murata and Ai Sakamoto and Yuko Wada and Katsushi Yamaguchi and Yuriko Osakabe and Keishi Osakabe and Akira Kanno and Yukio Ozaki and Seiji Takayama",
note = "Funding Information: We thank A. Takahashi, F. Kodama, H. Asao and A. Akita for technical assistance, and T. Nishimoto and H. Asao for a kind gift of 3-year-old A. officinalis cv. Super Welcome. Computational resources were provided by the Data Integration and Analysis Facility, NIBB. This work was supported in part by Grants-in-Aid for Scientific Research on Innovative Areas (23113002, 16H06467 to S.T.), and Grants-in-Aid for Scientific Research (25252021, 16H06380 to S.T.) from the Ministry of Education, Culture, Sports, Science and Technology of Japan (MEXT), Takeda Science Foundation (to K.M.), and the Council for Science, Technology and Innovation (CSTI), Cross-ministerial Strategic Innovation Promotion Program (SIP), {\textquoteleft}Technologies for creating next-generation agriculture, forestry and fisheries{\textquoteright} (funding agency: Bio-oriented Technology Research Advancement Institution, NARO) to K.O. A Grant-in-Aid for Science and technology research promotion program for agriculture, forestry, fisheries and food industry (No. 27002B) from the Ministry of Agriculture, Forestry and Fisheries (MAFF) (Japan) (to A.K. and Y. Ozaki). S.T. supervised this project. K.M. and S.T. designed the experiments. K.M., K.U., T.M. and A.S. carried out molecular experiments, with assistance from Y.W. S.F. carried out phylogenetic analysis. Y. Osakabe and K.O. were responsible for genome editing constructs. Y. Ozaki and A. K. collected and maintained the plant materials, observed the sex phenotypes and extracted the DNA. S.S. and K.Y. carried out genome sequencing and assembly. K.M. and S.T. wrote the manuscript, helped by S.S. and S.F., and all other authors contributed to editing. The MSE1 sequence and high-throughput sequencing data used in this study have been deposited in DNA Data Bank of Japan (DDBJ) under accession numbers LC190965, SAMD00047009, SAMD00047010, SAMD00047011, SAMD00047624 and SAMD00064393. Publisher Copyright: {\textcopyright} 2016 Molecular Biology Society of Japan and John Wiley & Sons Australia, Ltd",
year = "2017",
month = jan,
day = "1",
doi = "10.1111/gtc.12453",
language = "English",
volume = "22",
pages = "115--123",
journal = "Genes to Cells",
issn = "1356-9597",
publisher = "Wiley-Blackwell",
number = "1",
}