A new mutation analysis system was developed by utilizing a DNA intercalator in reversed micelles. The hybridization signal was amplified by monitoring the intercalation behavior between a porphirine derivative and a double strand DNA in reversed micelles. Hybridization rate in reversed micelles was analyzed for three sample DNAs bearing a mutation in a different part. The location of the mutation significantly affected the hybridization behavior. This novel method allows the DNA concentration to be reduced to less than one-tenth of that employed in the conventional reversed micellar method.
All Science Journal Classification (ASJC) codes
- General Chemistry
- General Chemical Engineering