Mutation analysis of the putative tumor suppressor PTEN/MMAC1 in human ovarian cancer

Akira Yokomizo; Donald J. Tindall; Lynn Hartmann; Robert B. Jenkins; David I. Smith; Wanguo Liu

doi:10.3892/ijo.13.1.101

Mutation analysis of the putative tumor suppressor PTEN/MMAC1 in human ovarian cancer

Akira Yokomizo, Donald J. Tindall, Lynn Hartmann, Robert B. Jenkins, David I. Smith, Wanguo Liu

Research output: Contribution to journal › Article › peer-review

33 Citations (Scopus)

Abstract

A putative tumor suppressor, PTEN/MMAC1 gene was recently identified at 10q23 and was found to be frequently altered in several types of human tumors. To determine the possible role of this gene in human ovarian tumors, we screened 31 unselected primary epithelial ovarian cancers with matched normal tissues and 7 ovarian cancer cell lines. Two homozygous deletions (4.8%) were detected in primary ovarian tumors by a semi-quantitative multiplex polymerase chain reaction. However, no point mutations were observed in any of the PTEN/MMAC1 coding sequences as determined by denaturing high-performance liquid chromatography and DNA sequencing. These data indicate that PTEN/MMAC1 mutations are rare in ovarian cancers.

Original language	English
Pages (from-to)	101-105
Number of pages	5
Journal	International journal of oncology
Volume	13
Issue number	1
DOIs	https://doi.org/10.3892/ijo.13.1.101
Publication status	Published - Jul 1998
Externally published	Yes

All Science Journal Classification (ASJC) codes

Oncology
Cancer Research

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10.3892/ijo.13.1.101

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AU - Smith, David I.

AU - Liu, Wanguo

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Mutation analysis of the putative tumor suppressor PTEN/MMAC1 in human ovarian cancer

Abstract

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