TY - JOUR
T1 - Mushroom extract inhibits ultraviolet B-induced cellular senescence in human keratinocytes
AU - Chong, Zhao
AU - Matsuo, Haruka
AU - Kuroda, Mai
AU - Yamashita, Shuntaro
AU - Parajuli, Gopal Prasad
AU - Manandhar, Hira Kaji
AU - Shimizu, Kuniyoshi
AU - Katakura, Yoshinori
N1 - Publisher Copyright:
© 2018, Springer Science+Business Media B.V., part of Springer Nature.
PY - 2018/6/1
Y1 - 2018/6/1
N2 - Mushrooms possess various bioactivities and are used as nutritional supplements and medicinal products. Twenty-nine bioactive components have been extracted recently from mushrooms grown in Nepal. In this study, we evaluated the ability of these mushroom extracts to augment SIRT1, a mammalian SIR2 homologue localized in cytosol and nuclei. We established a system for screening food ingredients that augment the SIRT1 promoter in HaCaT cells, and identified a SIRT1-augmenting mushroom extract (number 28, Trametes versicolor). UVB irradiation induced cellular senescence in HaCaT cells, as evidenced by increased activity and expression of cellular senescence markers including senescence-associated β-galactosidase, p21, p16, phosphorylated p38, and γH2AX. Results clearly showed that the mushroom extract (No. 28) suppressed the ultraviolet B irradiation-induced cellular senescence in HaCaT cells possibly through augmenting SIRT1 expression.
AB - Mushrooms possess various bioactivities and are used as nutritional supplements and medicinal products. Twenty-nine bioactive components have been extracted recently from mushrooms grown in Nepal. In this study, we evaluated the ability of these mushroom extracts to augment SIRT1, a mammalian SIR2 homologue localized in cytosol and nuclei. We established a system for screening food ingredients that augment the SIRT1 promoter in HaCaT cells, and identified a SIRT1-augmenting mushroom extract (number 28, Trametes versicolor). UVB irradiation induced cellular senescence in HaCaT cells, as evidenced by increased activity and expression of cellular senescence markers including senescence-associated β-galactosidase, p21, p16, phosphorylated p38, and γH2AX. Results clearly showed that the mushroom extract (No. 28) suppressed the ultraviolet B irradiation-induced cellular senescence in HaCaT cells possibly through augmenting SIRT1 expression.
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U2 - 10.1007/s10616-018-0229-1
DO - 10.1007/s10616-018-0229-1
M3 - Article
AN - SCOPUS:85047914454
SN - 0920-9069
VL - 70
SP - 1001
EP - 1008
JO - Cytotechnology
JF - Cytotechnology
IS - 3
ER -