Mushroom extract inhibits ultraviolet B-induced cellular senescence in human keratinocytes

Zhao Chong, Haruka Matsuo, Mai Kuroda, Shuntaro Yamashita, Gopal Prasad Parajuli, Hira Kaji Manandhar, Kuniyoshi Shimizu, Yoshinori Katakura

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)


Mushrooms possess various bioactivities and are used as nutritional supplements and medicinal products. Twenty-nine bioactive components have been extracted recently from mushrooms grown in Nepal. In this study, we evaluated the ability of these mushroom extracts to augment SIRT1, a mammalian SIR2 homologue localized in cytosol and nuclei. We established a system for screening food ingredients that augment the SIRT1 promoter in HaCaT cells, and identified a SIRT1-augmenting mushroom extract (number 28, Trametes versicolor). UVB irradiation induced cellular senescence in HaCaT cells, as evidenced by increased activity and expression of cellular senescence markers including senescence-associated β-galactosidase, p21, p16, phosphorylated p38, and γH2AX. Results clearly showed that the mushroom extract (No. 28) suppressed the ultraviolet B irradiation-induced cellular senescence in HaCaT cells possibly through augmenting SIRT1 expression.

Original languageEnglish
Pages (from-to)1001-1008
Number of pages8
Issue number3
Publication statusPublished - Jun 1 2018

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Biomedical Engineering
  • Clinical Biochemistry
  • Cell Biology


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