Multimodal imaging of living cells with multiplex coherent anti-stokes Raman scattering (CARS), third-order sum frequency generation (TSFG) and two-photon excitation fluorescence (TPEF) using a nanosecond white-light laser source

Hiroki Segawa; Masanari Okuno; Philippe Leproux; Vincent Couderc; Takeaki Ozawa; Hideaki Kano

doi:10.2116/analsci.31.299

Multimodal imaging of living cells with multiplex coherent anti-stokes Raman scattering (CARS), third-order sum frequency generation (TSFG) and two-photon excitation fluorescence (TPEF) using a nanosecond white-light laser source

Hiroki Segawa, Masanari Okuno, Philippe Leproux, Vincent Couderc, Takeaki Ozawa, Hideaki Kano

Research output: Contribution to journal › Article › peer-review

12 Citations (Scopus)

Abstract

The subnanosecond "white-light laser" source has been applied to multimodal, multiphoton, and multiplex spectroscopic imaging (M ³ spectroscopic imaging) with coherent anti-Stokes Raman scattering (CARS), third-order sum frequency generation (TSFG), and two-photon excitation fluorescence (TPEF). As the proof-of-principle experiment, we performed simultaneous imaging of polystyrene beads with TSFG and TPEF. This technique is then applied to live cell imaging. Mouse L929 fibroblastic cells are clearly visualized by CARS, TSFG, and TPEF processes. M ³ spectroscopic imaging provides various and unique cellular information with different image contrast based on each multiphoton process.

Original language	English
Pages (from-to)	299-305
Number of pages	7
Journal	analytical sciences
Volume	31
Issue number	4
DOIs	https://doi.org/10.2116/analsci.31.299
Publication status	Published - 2015
Externally published	Yes

All Science Journal Classification (ASJC) codes

Analytical Chemistry

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Segawa, H., Okuno, M., Leproux, P., Couderc, V., Ozawa, T., & Kano, H. (2015). Multimodal imaging of living cells with multiplex coherent anti-stokes Raman scattering (CARS), third-order sum frequency generation (TSFG) and two-photon excitation fluorescence (TPEF) using a nanosecond white-light laser source. analytical sciences, 31(4), 299-305. https://doi.org/10.2116/analsci.31.299

Multimodal imaging of living cells with multiplex coherent anti-stokes Raman scattering (CARS), third-order sum frequency generation (TSFG) and two-photon excitation fluorescence (TPEF) using a nanosecond white-light laser source. / Segawa, Hiroki; Okuno, Masanari; Leproux, Philippe et al.
In: analytical sciences, Vol. 31, No. 4, 2015, p. 299-305.

Research output: Contribution to journal › Article › peer-review

Segawa, H, Okuno, M, Leproux, P, Couderc, V, Ozawa, T & Kano, H 2015, 'Multimodal imaging of living cells with multiplex coherent anti-stokes Raman scattering (CARS), third-order sum frequency generation (TSFG) and two-photon excitation fluorescence (TPEF) using a nanosecond white-light laser source', analytical sciences, vol. 31, no. 4, pp. 299-305. https://doi.org/10.2116/analsci.31.299

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Multimodal imaging of living cells with multiplex coherent anti-stokes Raman scattering (CARS), third-order sum frequency generation (TSFG) and two-photon excitation fluorescence (TPEF) using a nanosecond white-light laser source

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