Multicolor multiphoton microscopy based on a nanosecond supercontinuum laser source

Claire Lefort, Rodney P. O'Connor, Véronique Blanquet, Laetitia Magnol, Hideaki Kano, Vincent Tombelaine, Philippe Lévêque, Vincent Couderc, Philippe Leproux

Research output: Contribution to journalArticlepeer-review

30 Citations (Scopus)


Multicolor multiphoton microscopy is experimentally demonstrated for the first time on a spectral bandwidth of excitation of 300 nm (full width half maximum) thanks to the implementation a nanosecond supercontinuum (SC) source compact and simple with a low repetition rate. The interest of such a wide spectral bandwidth, never demonstrated until now, is highlighted in vivo: images of glioma tumor cells stably expressing eGFP grafted on the brain of a mouse and its blood vessels network labelled with Texas Red(®) are obtained. These two fluorophores have a spectral bandwidth covering the whole 300 nm available. In parallel, a similar image quality is obtained on a sample of mouse muscle in vitro when excited with this nanosecond SC source or with a classical high rate, femtosecond and quasi monochromatic laser. This opens the way for (i) a simple and very complete biological characterization never performed to date with multiphoton processes, (ii) multiple means of contrast in nonlinear imaging allowed by the use of numerous fluorophores and (iii) other multiphoton processes like three-photon ones.

Original languageEnglish
Pages (from-to)709-714
Number of pages6
JournalJournal of Biophotonics
Issue number7
Publication statusPublished - Jul 1 2016
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • General Chemistry
  • General Materials Science
  • General Biochemistry,Genetics and Molecular Biology
  • General Engineering
  • General Physics and Astronomy


Dive into the research topics of 'Multicolor multiphoton microscopy based on a nanosecond supercontinuum laser source'. Together they form a unique fingerprint.

Cite this