TY - JOUR
T1 - Motility of Microtubules on the Inner Surface of Water-in-Oil Emulsion Droplets
AU - Tsuji, Mikako
AU - Rashedul Kabir, Arif Md
AU - Ito, Masaki
AU - Inoue, Daisuke
AU - Kokado, Kenta
AU - Sada, Kazuki
AU - Kakugo, Akira
N1 - Funding Information:
This study was financially supported by a Grant-in-Aid for Scientific Research on Innovative Areas (JSPS KAKENHI grant no. JP24104004) from the Ministry of Education, Culture, Sports, Science, and Technology of Japan.
Publisher Copyright:
© 2017 American Chemical Society.
PY - 2017/10/31
Y1 - 2017/10/31
N2 - Water-in-oil emulsion systems have recently attracted much attention in various fields. However, functionalization of water-in-oil emulsion systems, which is required for expanding their applications in industries and research, has been challenging. We now demonstrate the functionalization of a water-in-oil emulsion system by anchoring a target protein molecule. A microtubule (MT)-associated motor protein kinesin-1 was successfully anchored to the inner surface of water-in-oil emulsion droplets by employing the specific interaction of nickel-nitrilotriacetic acid-histidine tag. The MTs exhibited a gliding motion on the kinesin-functionalized inner surface of the emulsion droplets, which confirmed the success of the functionalization of the water-in-oil emulsion system. This result would be beneficial in exploring the roles of biomolecular motor systems in the cellular events that take place at the cell membrane and might also contribute to expanding the nanotechnological applications of biomolecular motors and water-in-oil emulsion systems in the future.
AB - Water-in-oil emulsion systems have recently attracted much attention in various fields. However, functionalization of water-in-oil emulsion systems, which is required for expanding their applications in industries and research, has been challenging. We now demonstrate the functionalization of a water-in-oil emulsion system by anchoring a target protein molecule. A microtubule (MT)-associated motor protein kinesin-1 was successfully anchored to the inner surface of water-in-oil emulsion droplets by employing the specific interaction of nickel-nitrilotriacetic acid-histidine tag. The MTs exhibited a gliding motion on the kinesin-functionalized inner surface of the emulsion droplets, which confirmed the success of the functionalization of the water-in-oil emulsion system. This result would be beneficial in exploring the roles of biomolecular motor systems in the cellular events that take place at the cell membrane and might also contribute to expanding the nanotechnological applications of biomolecular motors and water-in-oil emulsion systems in the future.
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U2 - 10.1021/acs.langmuir.7b01550
DO - 10.1021/acs.langmuir.7b01550
M3 - Article
AN - SCOPUS:85032647914
SN - 0743-7463
VL - 33
SP - 12108
EP - 12113
JO - Langmuir
JF - Langmuir
IS - 43
ER -