TY - JOUR
T1 - Monohydroxylated polycyclic aromatic hydrocarbons influence spicule formation in the early development of sea urchins (Hemicentrotus pulcherrimus)
AU - Suzuki, Nobuo
AU - Ogiso, Shouzo
AU - Yachiguchi, Koji
AU - Kawabe, Kimi
AU - Makino, Fumiya
AU - Toriba, Akira
AU - Kiyomoto, Masato
AU - Sekiguchi, Toshio
AU - Tabuchi, Yoshiaki
AU - Kondo, Takashi
AU - Kitamura, Kei Ichiro
AU - Hong, Chun Sang
AU - Srivastav, Ajai K.
AU - Oshima, Yuji
AU - Hattori, Atsuhiko
AU - Hayakawa, Kazuichi
N1 - Funding Information:
We are grateful to Dr. Takuya Minokawa (Research Center for Marine Biology, Graduate School of Life Sciences, Tohoku University) for information regarding PCR primers in the mRNA expression analysis. This study was supported in part by grants to N.S. ( Grant-in-Aid for Scientific Research [C] No. 24620004 by JSPS), to T.S. ( Grant-in-Aid for Young Scientists [B] No. 40378568 by JSPS), to Y.T. ( Grant-in-Aid for Scientific Research [B] No. 24310046 by JSPS), to K.K. ( Grant-in-Aid for Scientific Research [C] No. 24500848 by JSPS), to A.H. ( Grant-in-Aid for Scientific Research [C] No. 24570068 by JSPS), and to K.H. ( Grant-in-Aid for Exploratory Research No. 24651044 by JSPS). This study was performed under the cooperative research program of the Institute of Nature and Environmental Technology, Kanazawa University (Accept No. 1).
Publisher Copyright:
© 2015 Elsevier Inc. All rights reserved.
PY - 2015/6/1
Y1 - 2015/6/1
N2 - We previously demonstrated that monohydroxylated polycyclic aromatic hydrocarbons (OHPAHs), which are metabolites of polycyclic aromatic hydrocarbons (PAHs), act on calcified tissue and suppress osteoblastic and osteoclastic activity in the scales of teleost fish. The compounds may possibly influence other calcified tissues. Thus, the present study noted the calcified spicules in sea urchins and examined the effect of both PAHs and OHPAHs on spicule formation during the embryogenesis of sea urchins. After fertilization, benz[a]anthracene (BaA) and 4-hydroxybenz[a]anthracene (4-OHBaA) were added to seawater at concentrations of 10- 8 and 10- 7 M and kept at 18 °C. The influence of the compound was given at the time of the pluteus larva. At this stage, the length of the spicule was significantly suppressed by 4-OHBaA (10- 8 and 10- 7 M). BaA (10- 7 M) decreased the length of the spicule significantly, while the length did not change with BaA (10- 8 M). The expression of mRNAs (spicule matrix protein and transcription factors) in the 4-OHBaA (10- 7 M)-treated embryos was more strongly inhibited than were those in the BaA (10- 7 M)-treated embryos. This is the first study to demonstrate that OHPAHs suppress spicule formation in sea urchins.
AB - We previously demonstrated that monohydroxylated polycyclic aromatic hydrocarbons (OHPAHs), which are metabolites of polycyclic aromatic hydrocarbons (PAHs), act on calcified tissue and suppress osteoblastic and osteoclastic activity in the scales of teleost fish. The compounds may possibly influence other calcified tissues. Thus, the present study noted the calcified spicules in sea urchins and examined the effect of both PAHs and OHPAHs on spicule formation during the embryogenesis of sea urchins. After fertilization, benz[a]anthracene (BaA) and 4-hydroxybenz[a]anthracene (4-OHBaA) were added to seawater at concentrations of 10- 8 and 10- 7 M and kept at 18 °C. The influence of the compound was given at the time of the pluteus larva. At this stage, the length of the spicule was significantly suppressed by 4-OHBaA (10- 8 and 10- 7 M). BaA (10- 7 M) decreased the length of the spicule significantly, while the length did not change with BaA (10- 8 M). The expression of mRNAs (spicule matrix protein and transcription factors) in the 4-OHBaA (10- 7 M)-treated embryos was more strongly inhibited than were those in the BaA (10- 7 M)-treated embryos. This is the first study to demonstrate that OHPAHs suppress spicule formation in sea urchins.
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U2 - 10.1016/j.cbpc.2015.02.004
DO - 10.1016/j.cbpc.2015.02.004
M3 - Article
C2 - 25737366
AN - SCOPUS:84927556854
SN - 1532-0456
VL - 171
SP - 55
EP - 60
JO - Comparative Biochemistry and Physiology Part - C: Toxicology and Pharmacology
JF - Comparative Biochemistry and Physiology Part - C: Toxicology and Pharmacology
ER -