TY - JOUR
T1 - Monoclonal antibody based enzyme immunoassay for marihuana (cannabinoid) compounds
AU - Tanaka, Hiroyuki
AU - Goto, Yuri
AU - Shoyama, Yukihiro
PY - 1996
Y1 - 1996
N2 - MAb against Δ1-THCA was produced by fusing hybridoma with splenocytes immunized with Δ1-THCA-BSA conjugate and hypoxanthine, aminopterine, thymidine-sensitive mouse myeloma cell line, P3-X63-Ag8-653. The cross- reaction of anti-Δ1-THCA antibody against other cannabinoids was very wide, thus many cannabinoids and a spiro-compound were reactive suggesting that 2'- hydroxyl, 6'-hydroxyl or 6'-O-alkyl, 4'-alkylbenzene ring moiety is necessary for its reactivity. It became evident that this ELISA was able to be applied to the biotransformation experiments of cannabinoids in plant tissue culture system. The metabolites of Δ6-THC such as two major metabolites. 7-oxo- Δ6-THC and 7-hydroxyl-Δ6-THC were also detectable by this ELISA.
AB - MAb against Δ1-THCA was produced by fusing hybridoma with splenocytes immunized with Δ1-THCA-BSA conjugate and hypoxanthine, aminopterine, thymidine-sensitive mouse myeloma cell line, P3-X63-Ag8-653. The cross- reaction of anti-Δ1-THCA antibody against other cannabinoids was very wide, thus many cannabinoids and a spiro-compound were reactive suggesting that 2'- hydroxyl, 6'-hydroxyl or 6'-O-alkyl, 4'-alkylbenzene ring moiety is necessary for its reactivity. It became evident that this ELISA was able to be applied to the biotransformation experiments of cannabinoids in plant tissue culture system. The metabolites of Δ6-THC such as two major metabolites. 7-oxo- Δ6-THC and 7-hydroxyl-Δ6-THC were also detectable by this ELISA.
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U2 - 10.1080/01971529608005796
DO - 10.1080/01971529608005796
M3 - Article
C2 - 8957763
AN - SCOPUS:0029852161
SN - 0197-1522
VL - 17
SP - 321
EP - 342
JO - Journal of Immunoassay
JF - Journal of Immunoassay
IS - 4
ER -