Monitoring of human herpesviruses after allogeneic peripheral blood stem cell transplantation and bone marrow transplantation

Yoshinobu Maeda, Takanori Teshima, Masao Yamada, Katsuji Shinagawa, Shinji Nakao, Yuju Ohno, Kensuke Kojima, Masamichi Hara, Koji Nagafuji, Shin Hayashi, Shunnichi Fukuda, Hitoshi Sawada, Kosei Matsue, Katsuto Takenaka, Fumihiko Ishimaru, Kazuma Ikeda, Kenji Niiya, Mine Harada

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71 Citations (Scopus)


Herpesviruses frequently cause serious complications after allogeneic bone marrow transplantation (allo-BMT). Recent studies have shown more rapid immune reconstitution after allogeneic peripheral blood stem cell transplantation (allo-PBSCT) compared with allo-BMT. However, it has not been clarified whether the improved immune reconstitution after allo-PBSCT is associated with a lower incidence of herpesvirus infections. We monitored the emergence of Epstein-Barr virus (EBV), cytomegalovirus (CMV), human herpesvirus 6 (HHV-6) and HHV-7 DNA by a nested-double polymerase chain reaction in peripheral blood leucocytes from 22 allo-BMT and 16 allo-PBSCT patients. Each virus had an unique temporal profile of detection. HHV-6 DNA was detected most frequently at 3 weeks after transplantation, whereas CMV and EBV DNA were detected later (2-3 months). Detection rates of HHV-6 DNA at 3 and 4 weeks after allo-BMT were significantly higher than those after allo- PBSCT (9/16 v 2/13 at 3 weeks. P < 0.01; 10/21 v 1/15 at 4 weeks, P < 0.01). Detection rates of the other three herpesviruses after the two types of allogeneic transplantation were not significantly different throughout observation period. Furthermore, detection of HHV-6 DNA within the first 4 weeks was associated with delayed platelet engraftment after both allo-BMT and allo-PBSCT (P<0.01). These results suggest an advantage for allo-PBSCT over allo-BMT in terms of suppression of HHV-6 reactivation and prevention of subsequent complications.

Original languageEnglish
Pages (from-to)295-302
Number of pages8
JournalBritish Journal of Haematology
Issue number1
Publication statusPublished - 1999
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Hematology


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