Molecular imprinting technique in putrescine visualized detection

Xiaoguang Ying, Hiro Taka Yoshioka, Chuanjun Liu, Fumihiro Sassa, Kenshi Hayashi

Research output: Contribution to journalArticlepeer-review

18 Citations (Scopus)


This work is an exploration into visualizing measurement of putrescine specific adsorption by photographing colorized molecule imprinting chromogenic hydrogel. Membranes are prepared with imitate templates 1,4-butylene glycol, adipic acid or succinic acid, respectively and ninhydrin is used as chromogenic agent for target molecule putrescine. The adsorbing concentration on MIP is reflected in the form of visible violet-colored stain differed in shape and shade, which are recorded by hyper spectral camera and proceeded into intuitive 3D isohypse surfaces plot. By quantifying the height of surface peaks, imprinting efficiency is estimated in accordance with polyvinyl alcohol concentration and crosslinking degree. The imprinting efficiency of three imitate molecule templates is also discussed.

Original languageEnglish
Pages (from-to)870-880
Number of pages11
JournalSensors and Actuators, B: Chemical
Publication statusPublished - Apr 1 2018

All Science Journal Classification (ASJC) codes

  • Electronic, Optical and Magnetic Materials
  • Instrumentation
  • Condensed Matter Physics
  • Surfaces, Coatings and Films
  • Metals and Alloys
  • Electrical and Electronic Engineering
  • Materials Chemistry


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