TY - JOUR
T1 - Molecular evolution of amphioxus fructose-1,6 bisphosphate aldolase
AU - Kuba, Masako
AU - Yatsuki, Hitomi
AU - Kusakabe, Takahiro
AU - Takasaki, Yozo
AU - Nikoh, Naruo
AU - Miyata, Takashi
AU - Yamaguchi, Takao
AU - Hori, Katsuji
N1 - Funding Information:
1This work was supported in part by a Grant-in-Aid for Scienti®c Research from the Ministry of Education, Science, Sports and Culture of Japan.
PY - 1997/12/15
Y1 - 1997/12/15
N2 - The cDNA for amphioxus fructose-1,6-bisphosphate (FBP)-aldolase was isolated and its nucleotide sequence was determined. In the cDNA, there existed a probable open reading frame comprising 1080 bp; hence, 359 amino acid residues were deduced. The amino acid sequence indicates the deletion of 4 residues from N-terminus, in comparison with the sequence of FBP-aldolase isozymes from other sources. There was only one FBP-aldolase gene, and one enzyme species corresponding, in the amphioxus; this is the first report of the existence of a single FBP-aldolase species in animals. Enzymatic studies of both native and the recombinant FBP-aldolase suggest that the amphioxus enzyme belongs to an ancestral class I type which is not discovered among vertebrate aldolase isozymes.
AB - The cDNA for amphioxus fructose-1,6-bisphosphate (FBP)-aldolase was isolated and its nucleotide sequence was determined. In the cDNA, there existed a probable open reading frame comprising 1080 bp; hence, 359 amino acid residues were deduced. The amino acid sequence indicates the deletion of 4 residues from N-terminus, in comparison with the sequence of FBP-aldolase isozymes from other sources. There was only one FBP-aldolase gene, and one enzyme species corresponding, in the amphioxus; this is the first report of the existence of a single FBP-aldolase species in animals. Enzymatic studies of both native and the recombinant FBP-aldolase suggest that the amphioxus enzyme belongs to an ancestral class I type which is not discovered among vertebrate aldolase isozymes.
UR - http://www.scopus.com/inward/record.url?scp=0031574451&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0031574451&partnerID=8YFLogxK
U2 - 10.1006/abbi.1997.0384
DO - 10.1006/abbi.1997.0384
M3 - Article
C2 - 9434745
AN - SCOPUS:0031574451
SN - 0003-9861
VL - 348
SP - 329
EP - 336
JO - Archives of Biochemistry and Biophysics
JF - Archives of Biochemistry and Biophysics
IS - 2
ER -