TY - JOUR
T1 - Molecular evidence for the existence of two distinct IL-8 lineages of teleost CXC-chemokines
AU - Abdelkhalek, Nevien K.
AU - Komiya, Asuka
AU - Kato-Unoki, Yoko
AU - Somamoto, Tomonori
AU - Nakao, Miki
N1 - Funding Information:
The authors thank Dr. Masakazu Kondo, National Fisheries University, Japan, for providing experimental fish, and Dr. Valerie J. Smith, University of St. Andrews, for English editing. Mr. Shiro Sameshima provided a technical assistance in the cDNA library construction. This study was supported, in part, by a grant-in-aid from the Ministry of Education, Culture, Sports, Science and Technology, Japan, to MN. NKA is supported by a scholarship from the Ministry of Higher Education, Egypt.
PY - 2009/12
Y1 - 2009/12
N2 - Interleukin-8 (IL-8) is a CXC-type chemokine with a chemotactic activity mainly on neutrophils and plays a key role in promoting inflammation. In teleosts, several CXC-chemokines have been cloned and characterized as being IL-8-like. Phylogenetic data however indicate that the reported teleost IL-8-like chemokines are substantially remote from mammalian IL-8, forming a fish-specific clade of IL-8-like chemokines distinct from that of tetrapod IL-8. In the present study, a novel IL-8-like chemokine, designated CaIL-8, has been found in the expressed sequence tags of carp gills and identified as an orthologue of mammalian IL-8. The CaIL-8 transcript encodes 99 amino acids containing a typical CXC motif but lacks an ELR motif, as in most teleost IL-8-like chemokines. Phylogenetic tree constructed by the maximum likelihood method suggests a closer relationship of CaIL-8 with mammalian IL-8 than with other teleost CXC-chemokines reported to be IL-8-like. In a normal unstimulated carp, CaIL-8 mRNA was detected by RT-PCR only in gills, kidney, spleen, heart and peripheral blood leukocytes, in contrast to a previously reported carp IL-8-like chemokine CXCa, which shows ubiquitous basal expression. The results, taken together, are strongly indicative of the presence of two major IL-8-like lineages of CXC-chemokines in teleost.
AB - Interleukin-8 (IL-8) is a CXC-type chemokine with a chemotactic activity mainly on neutrophils and plays a key role in promoting inflammation. In teleosts, several CXC-chemokines have been cloned and characterized as being IL-8-like. Phylogenetic data however indicate that the reported teleost IL-8-like chemokines are substantially remote from mammalian IL-8, forming a fish-specific clade of IL-8-like chemokines distinct from that of tetrapod IL-8. In the present study, a novel IL-8-like chemokine, designated CaIL-8, has been found in the expressed sequence tags of carp gills and identified as an orthologue of mammalian IL-8. The CaIL-8 transcript encodes 99 amino acids containing a typical CXC motif but lacks an ELR motif, as in most teleost IL-8-like chemokines. Phylogenetic tree constructed by the maximum likelihood method suggests a closer relationship of CaIL-8 with mammalian IL-8 than with other teleost CXC-chemokines reported to be IL-8-like. In a normal unstimulated carp, CaIL-8 mRNA was detected by RT-PCR only in gills, kidney, spleen, heart and peripheral blood leukocytes, in contrast to a previously reported carp IL-8-like chemokine CXCa, which shows ubiquitous basal expression. The results, taken together, are strongly indicative of the presence of two major IL-8-like lineages of CXC-chemokines in teleost.
UR - https://www.scopus.com/pages/publications/70350786395
UR - https://www.scopus.com/pages/publications/70350786395#tab=citedBy
U2 - 10.1016/j.fsi.2009.08.004
DO - 10.1016/j.fsi.2009.08.004
M3 - Article
C2 - 19699802
AN - SCOPUS:70350786395
SN - 1050-4648
VL - 27
SP - 763
EP - 767
JO - Fish and Shellfish Immunology
JF - Fish and Shellfish Immunology
IS - 6
ER -