TY - JOUR
T1 - Molecular detection of maturation stages in the developing kidney
AU - Naganuma, Hidekazu
AU - Miike, Koichiro
AU - Ohmori, Tomoko
AU - Tanigawa, Shunsuke
AU - Ichikawa, Takumi
AU - Yamane, Mariko
AU - Eto, Masatoshi
AU - Niwa, Hitoshi
AU - Kobayashi, Akio
AU - Nishinakamura, Ryuichi
N1 - Funding Information:
We thank Sayoko Fujimura, Shingo Usuki, and Itoshi Nikaido for technical assistance and advice. We also thank Alison Sherwin, PhD, from Edanz Group ( https://en-author-services.edanzgroup.com/ac ) for editing a draft of this manuscript. The study was supported, in part, by a KAKENHI grant ( JP17H06177 to R.N.) from the Japan Society for the Promotion of Science .
Funding Information:
We thank Sayoko Fujimura, Shingo Usuki, and Itoshi Nikaido for technical assistance and advice. We also thank Alison Sherwin, PhD, from Edanz Group (https://en-author-services.edanzgroup.com/ac) for editing a draft of this manuscript. The study was supported, in part, by a KAKENHI grant (JP17H06177 to R.N.) from the Japan Society for the Promotion of Science.
Publisher Copyright:
© 2020 The Authors
PY - 2021/2
Y1 - 2021/2
N2 - Recent advances in stem cell biology have enabled the generation of kidney organoids in vitro, and further maturation of these organoids is observed after experimental transplantation. However, the current organoids remain immature and their precise maturation stages are difficult to determine because of limited information on developmental stage-dependent gene expressions in the kidney in vivo. To establish relevant molecular coordinates, we performed single-cell RNA sequencing (scRNA-seq) on developing kidneys at different stages in the mouse. By selecting genes that exhibited upregulation at birth compared with embryonic day 15.5 as well as cell lineage-specific expression, we generated gene lists correlated with developmental stages in individual cell lineages. Application of these lists to transplanted embryonic kidneys revealed that most cell types, other than the collecting ducts, exhibited similar maturation to kidneys at the neonatal stage in vivo, revealing non-synchronous maturation across the cell lineages. Thus, our scRNA-seq data can serve as useful molecular coordinates to assess the maturation of developing kidneys and eventually of kidney organoids.
AB - Recent advances in stem cell biology have enabled the generation of kidney organoids in vitro, and further maturation of these organoids is observed after experimental transplantation. However, the current organoids remain immature and their precise maturation stages are difficult to determine because of limited information on developmental stage-dependent gene expressions in the kidney in vivo. To establish relevant molecular coordinates, we performed single-cell RNA sequencing (scRNA-seq) on developing kidneys at different stages in the mouse. By selecting genes that exhibited upregulation at birth compared with embryonic day 15.5 as well as cell lineage-specific expression, we generated gene lists correlated with developmental stages in individual cell lineages. Application of these lists to transplanted embryonic kidneys revealed that most cell types, other than the collecting ducts, exhibited similar maturation to kidneys at the neonatal stage in vivo, revealing non-synchronous maturation across the cell lineages. Thus, our scRNA-seq data can serve as useful molecular coordinates to assess the maturation of developing kidneys and eventually of kidney organoids.
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U2 - 10.1016/j.ydbio.2020.11.002
DO - 10.1016/j.ydbio.2020.11.002
M3 - Article
C2 - 33197428
AN - SCOPUS:85096357454
SN - 0012-1606
VL - 470
SP - 62
EP - 73
JO - Developmental Biology
JF - Developmental Biology
ER -