Molecular design of heteroprotein assemblies providing a bionanocup as a chemical reactor

Tomomi Koshiyama, Norihiko Yokoi, Takafumi Ueno, Snuji Kanamaru, Shingo Nagano, Yoshitsugu Shiro, Fumio Arisaka, Yoshihito Watanabe

Research output: Contribution to journalArticlepeer-review

25 Citations (Scopus)


The use of heteroprotein assemblies for providing a bionanocup to construct catalysts, sensors, and materials with novel properties, is discussed. It is used as a catalytic reaction space that consists of a heteroprotein assembly, which is a trimer of gene product 27 and gene product 5, isolated from bacteriophase T4. The bionanocup can be used for the accommodation of metal-porphyrin complexes by covalent linkage with cysteinyl thiols and its activity as an oxidation catalyst. Inductively coupled plasma (ICP) atomic-emission measurement and biocinchonianate (BCA) assay were used to observe the number of atoms in the reactor. The electron density map of derivatives shows a clear density between the male-amide moiety of FePP-MI and N7C. The proportions of Fe atoms to protein in the bionanocup were determined using the Vista-PRO (VARIAN) and bicinchonianate (BCA) methods.

Original languageEnglish
Pages (from-to)50-54
Number of pages5
Issue number1
Publication statusPublished - Jan 2008
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Engineering (miscellaneous)
  • General Chemistry
  • General Materials Science
  • Biotechnology
  • Biomaterials


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