TY - GEN
T1 - Molecular crowding effect on enzymatic reaction in a fl-microchamber to mimic crowded intracellular environment
AU - Murahara, H.
AU - Kaji, N.
AU - Tokeshi, M.
AU - Baba, Y.
N1 - Publisher Copyright:
© 2007 CBMS.
PY - 2007
Y1 - 2007
N2 - In this work, we investigated how the size of reaction space would affect enzyme activities to understand intracellular enzyme kinetics. Two approaches were used to create pseudo-intracellular environments: a high concentration of polymers in bulk state and microfabricated chambers. Both approaches were carefully compared and we found that an enzyme activity was inhibited in 7.2 fL chamber by molecular crowding effect as in the case of highly concentrated polymer solution.
AB - In this work, we investigated how the size of reaction space would affect enzyme activities to understand intracellular enzyme kinetics. Two approaches were used to create pseudo-intracellular environments: a high concentration of polymers in bulk state and microfabricated chambers. Both approaches were carefully compared and we found that an enzyme activity was inhibited in 7.2 fL chamber by molecular crowding effect as in the case of highly concentrated polymer solution.
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M3 - Conference contribution
AN - SCOPUS:84901793439
T3 - Proceedings of the 11th International Conference on Miniaturized Systems for Chemistry and Life Sciences, uTAS 2007
SP - 1003
EP - 1005
BT - Proceedings of the 11th International Conference on Miniaturized Systems for Chemistry and Life Sciences, uTAS 2007
A2 - Viovy, Jean-Louis
A2 - Tabeling, Patrick
A2 - Descroix, Stephanie
A2 - Malaquin, Laurent
PB - Chemical and Biological Microsystems Society
T2 - 11th International Conference on Miniaturized Systems for Chemistry and Life Sciences, uTAS 2007
Y2 - 7 October 2007 through 11 October 2007
ER -