TY - JOUR
T1 - Molecular cloning of rat leukemia inhibitory factor receptor α-chain gene and its expression during pregnancy
AU - Aikawa, Jun ichi
AU - Ikeda-Naiki, Sakae
AU - Ohgane, Jun
AU - Min, Kwan Sik
AU - Imamura, Takuya
AU - Sasai, Ken
AU - Shiota, Kunio
AU - Ogawa, Tomoya
N1 - Funding Information:
We thank the RIKEN GENE BANK for supplying DNA, Dr. M.M. Palcic (University of Alberta, Canada) for helpful suggestions regarding this manuscript and Ms. A. Takahashi (RIKEN) for her excellent secretarial assistance. This study was supported in part by a Grant-in-Aid from the Ministry of Education, Science and Culture, Japan.
PY - 1997/9/12
Y1 - 1997/9/12
N2 - Leukemia inhibitory factor (LIF) is a secreted glycoprotein and a pluripotent growth factor that acts on diverse cell systems. LIF transmits its effects via binding to transmembrane receptors, of which both high- and low-affinity forms have been identified. In this study, we analyzed the structure and expression of rat LIF receptor α-chain (rLIFR α) cDNA. A full-length clone of the cDNA encoding the membrane-bound form of rLIFR α protein was prepared by a combination of LA-PCR and 5' RACE using DNA reverse-transcribed from total RNA isolated from the livers of day-12 and day-14 pregnant rats as templates. The nucleotide sequence of a full-length clone was determined and further confirmed by analysis of shorter DNA fragment prepared by PCR using pfu polymerase. The gene for rLIFR α encodes a 1093 amine acid residue protein. The rLIFR α protein shows a high degree of similarity to mouse and human LIF receptor α-chain protein (89% and 76% amino acid sequence identifies, respectively). Only one molecular species of mRNA for the rLIFR α gene was detected in the liver and placenta. rLIFR α was expressed in liver of both non-pregnant and pregnant rats. The level of mRNA for the rLIFR α gene in placenta was maximum on day 16 of pregnancy.
AB - Leukemia inhibitory factor (LIF) is a secreted glycoprotein and a pluripotent growth factor that acts on diverse cell systems. LIF transmits its effects via binding to transmembrane receptors, of which both high- and low-affinity forms have been identified. In this study, we analyzed the structure and expression of rat LIF receptor α-chain (rLIFR α) cDNA. A full-length clone of the cDNA encoding the membrane-bound form of rLIFR α protein was prepared by a combination of LA-PCR and 5' RACE using DNA reverse-transcribed from total RNA isolated from the livers of day-12 and day-14 pregnant rats as templates. The nucleotide sequence of a full-length clone was determined and further confirmed by analysis of shorter DNA fragment prepared by PCR using pfu polymerase. The gene for rLIFR α encodes a 1093 amine acid residue protein. The rLIFR α protein shows a high degree of similarity to mouse and human LIF receptor α-chain protein (89% and 76% amino acid sequence identifies, respectively). Only one molecular species of mRNA for the rLIFR α gene was detected in the liver and placenta. rLIFR α was expressed in liver of both non-pregnant and pregnant rats. The level of mRNA for the rLIFR α gene in placenta was maximum on day 16 of pregnancy.
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U2 - 10.1016/S0167-4781(97)00079-1
DO - 10.1016/S0167-4781(97)00079-1
M3 - Article
C2 - 9349722
AN - SCOPUS:0030868491
SN - 0167-4781
VL - 1353
SP - 266
EP - 276
JO - Biochimica et Biophysica Acta - Gene Structure and Expression
JF - Biochimica et Biophysica Acta - Gene Structure and Expression
IS - 3
ER -