TY - JOUR
T1 - Molecular cloning and expression analysis of GFRα-3, a novel cDNA related to GDNFRα and NTNRα
AU - Nomoto, Satoshi
AU - Ito, Sachiko
AU - Yang, Li Xia
AU - Kiuchi, Kazutoshi
N1 - Funding Information:
We thank Dr. Kotaro Fujimoto (Department of Cellular and Molecular Biology, Kanazawa University) for useful discussions. This study is supported by the Frontier Research Program of RIKEN.
PY - 1998/3/27
Y1 - 1998/3/27
N2 - Glial-cell-line-derived neurotrophic factor (GDNF) and neurturin (NTN) are structurally related to TGF-β and are survival factors for sympathetic, sensory, and central nervous system neurons. GDNF transmits its signal primarily through a receptor complex containing the receptor tyrosine kinase Ret and a glycosyl-phosphatidylinositol (GPI)-linked receptor, GDNFRα. NTN utilizes a receptor complex system that consists of Ret and another GPI-linked receptor, NTNRα. We have identified a mouse cDNA, termed GFRα-3, that encodes a putative GPI-linked receptor. At the protein level, mouse GFRα-3 is 35% identical to mouse GDNFRα and 36% identical to mouse NTNRα. Northern blot analysis showed that GFRα-3 is expressed in fetal mouse heart, brain, lung, and kidney and adult heart. These results indicate that the tissue distribution of GFRα-3 mRNA is different from that of GDNFRα or NTNRα mRNA, and suggest that GFRα-3 may function in differentiation of embryonic cells expressing its mRNA.
AB - Glial-cell-line-derived neurotrophic factor (GDNF) and neurturin (NTN) are structurally related to TGF-β and are survival factors for sympathetic, sensory, and central nervous system neurons. GDNF transmits its signal primarily through a receptor complex containing the receptor tyrosine kinase Ret and a glycosyl-phosphatidylinositol (GPI)-linked receptor, GDNFRα. NTN utilizes a receptor complex system that consists of Ret and another GPI-linked receptor, NTNRα. We have identified a mouse cDNA, termed GFRα-3, that encodes a putative GPI-linked receptor. At the protein level, mouse GFRα-3 is 35% identical to mouse GDNFRα and 36% identical to mouse NTNRα. Northern blot analysis showed that GFRα-3 is expressed in fetal mouse heart, brain, lung, and kidney and adult heart. These results indicate that the tissue distribution of GFRα-3 mRNA is different from that of GDNFRα or NTNRα mRNA, and suggest that GFRα-3 may function in differentiation of embryonic cells expressing its mRNA.
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U2 - 10.1006/bbrc.1998.8361
DO - 10.1006/bbrc.1998.8361
M3 - Article
C2 - 9535755
AN - SCOPUS:0032571088
SN - 0006-291X
VL - 244
SP - 849
EP - 853
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 3
ER -