TY - JOUR
T1 - Molecular cloning and characterization of type III polyketide synthase from Plumbago zeylanica
AU - Sakamoto, Seiichi
AU - Morita, Yui
AU - Yusakul, Gorawit
AU - Putalun, Waraporn
AU - Tanaka, Hiroyuki
AU - Morimoto, Satoshi
N1 - Funding Information:
We appreciate Shota Muraki and Yui Terao for help with LC/ESI-MS/MS analysis. We also appreciate the technical support for sequence analysis from The Research Support Center, Research Center for Human Disease Modeling, Kyushu University Graduate School of Medical Sciences.
Publisher Copyright:
© 2020 Informa UK Limited, trading as Taylor & Francis Group.
PY - 2021
Y1 - 2021
N2 - Two cDNAs encoding type Ш polyketide synthase (PKS1) and chalcone synthase (CHS, PKS2), were cloned from fresh leaves of Plumbago zeylanica L. (P. zeylanica). Their heterologous expression revealed that PKS1 catalyzed the formation of five α-pyrones from three to six acetate units by accepting acetyl-CoA and malonyl-CoA. In contrast, PKS2 catalyzed the formation of naringenin and bisnoryangonin by accepting p-coumaroyl-CoA and malonyl-CoA. Naringenin is thought to be involved in the biosynthesis of various bioactive flavonoids. PKS2 can be used to molecular breeding to enhance the production of these useful secondary metabolites via its overexpression. (Figure presented.).
AB - Two cDNAs encoding type Ш polyketide synthase (PKS1) and chalcone synthase (CHS, PKS2), were cloned from fresh leaves of Plumbago zeylanica L. (P. zeylanica). Their heterologous expression revealed that PKS1 catalyzed the formation of five α-pyrones from three to six acetate units by accepting acetyl-CoA and malonyl-CoA. In contrast, PKS2 catalyzed the formation of naringenin and bisnoryangonin by accepting p-coumaroyl-CoA and malonyl-CoA. Naringenin is thought to be involved in the biosynthesis of various bioactive flavonoids. PKS2 can be used to molecular breeding to enhance the production of these useful secondary metabolites via its overexpression. (Figure presented.).
UR - http://www.scopus.com/inward/record.url?scp=85084851394&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85084851394&partnerID=8YFLogxK
U2 - 10.1080/10286020.2020.1754399
DO - 10.1080/10286020.2020.1754399
M3 - Article
C2 - 32400171
AN - SCOPUS:85084851394
SN - 1028-6020
VL - 23
SP - 478
EP - 490
JO - Journal of Asian Natural Products Research
JF - Journal of Asian Natural Products Research
IS - 5
ER -