TY - JOUR
T1 - Molecular characterisation of methicillin-resistant (MRSA) and methicillin-susceptible (MSSA) Staphylococcus aureus isolated from bovine subclinical mastitis and Egyptian raw milk cheese
AU - Zayda, Mahmoud Ge
AU - Masuda, Yoshimitsu
AU - Hammad, Ahmed M.
AU - Honjoh, Ken ichi
AU - Elbagory, Abdelrahman M.
AU - Miyamoto, Takahisa
N1 - Funding Information:
This work was collaboratively supported by the Missions sector, Egyptian Ministry of Higher Education and Research, and the laboratory of Food Hygienic Chemistry, Kyushu University , Japan.
Publisher Copyright:
© 2020 Elsevier Ltd
PY - 2020/5
Y1 - 2020/5
N2 - We investigated the characteristics of Staphylococcus aureus isolates causing bovine subclinical mastitis (SCM), and their genetic relatedness with the isolates obtained from Egyptian cheese. Twenty-five S. aureus isolates were identified from 150 SCM milk and 75 cheese samples. The antibiogram revealed multidrug-resistant (MDR) isolates. Fifteen isolates were categorised as methicillin-resistant. Antimicrobial resistance and virulence genes were detected. Spa typing and SCCmec classification were performed. More than 50% of isolates were found carrying human-specific virulence determinants, while other isolates characterised were presumed to be of bovine-origin. Spa-types t304, t688, t084 corresponded isolates from SCM milk and cheese samples; the similar genotypes from SCM and cheese displayed divergence in virulence traits. Moreover, our results revealed the novel spa-type t18546. Animals and dairy food could be a reservoir for transformative changes in S. aureus virulence, leading to the emergence of virulent MDR strains that may become potential public-health threats.
AB - We investigated the characteristics of Staphylococcus aureus isolates causing bovine subclinical mastitis (SCM), and their genetic relatedness with the isolates obtained from Egyptian cheese. Twenty-five S. aureus isolates were identified from 150 SCM milk and 75 cheese samples. The antibiogram revealed multidrug-resistant (MDR) isolates. Fifteen isolates were categorised as methicillin-resistant. Antimicrobial resistance and virulence genes were detected. Spa typing and SCCmec classification were performed. More than 50% of isolates were found carrying human-specific virulence determinants, while other isolates characterised were presumed to be of bovine-origin. Spa-types t304, t688, t084 corresponded isolates from SCM milk and cheese samples; the similar genotypes from SCM and cheese displayed divergence in virulence traits. Moreover, our results revealed the novel spa-type t18546. Animals and dairy food could be a reservoir for transformative changes in S. aureus virulence, leading to the emergence of virulent MDR strains that may become potential public-health threats.
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U2 - 10.1016/j.idairyj.2020.104646
DO - 10.1016/j.idairyj.2020.104646
M3 - Article
AN - SCOPUS:85079126963
SN - 0958-6946
VL - 104
JO - International Dairy Journal
JF - International Dairy Journal
M1 - 104646
ER -
