A cDNA encoding Zeta-class GST of the silkmoth, Bombyx mori (bmGSTZ), was cloned by a reverse transcriptase-polymerase chain reaction. The resulting clone was sequenced and deduced for amino acid sequence, which revealed 45-50% identities to Zeta-class GSTs from other organisms. A recombinant protein (rbmGSTZ) was functionally overexpressed in Bombyx mori cells in a soluble form and purified to homogeneity. rbmGSTZ was able to catalyze the biotranslation of glutathione with dichloroacetic acid. We found that the present GST-catalyzed dechlorination of permethrin and distributed abundantly in silkmoth strain exhibiting permethrin resistance. Our results suggest that bmGSTZ could contribute to permethrin resistance in lepidopteran.
All Science Journal Classification (ASJC) codes
- Agronomy and Crop Science
- Health, Toxicology and Mutagenesis