Modulatory effect of plasminogen on NMDA-induced increase in intracellular free calcium concentration in rat cultured hippocampal neurons

Kazuhide Inoue; Schuichi Koizumi; Kazuyuki Nakajima; Makoto Hamanoue; Shinichi Kohsaka

doi:10.1016/0304-3940(94)90941-5

Modulatory effect of plasminogen on NMDA-induced increase in intracellular free calcium concentration in rat cultured hippocampal neurons

Kazuhide Inoue, Schuichi Koizumi, Kazuyuki Nakajima, Makoto Hamanoue, Shinichi Kohsaka

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Abstract

The effect of plasminogen on the intracellular Ca²⁺ concentration ([Ca]_i) was examined in cultured rat hippocampal neurons. Plasminogen (100 nM) did not increase [Ca]_i in the presence of tetrodotoxin (TTX, 3 μM) but increased [Ca]_i in the absence of TTX. In all the cells which responded to plasminogen (100 nM), NMDA (5 μM) also increased [Ca]_i in the presence of TTX. Furthermore, plasminogen (100 nM) enhanced the NMDA-evoked [Ca]_i increase, and the potentiation by plasminogen was blocked by an NMDA receptor blocker, 2-amino-phosphonovalerate (APV). These data suggest that plasminogen enhances glutamate-evoked [Ca]_i increase through modulation of NMDA receptor in hippocampal neurons.

Original language	English
Pages (from-to)	87-90
Number of pages	4
Journal	Neuroscience Letters
Volume	179
Issue number	1-2
DOIs	https://doi.org/10.1016/0304-3940(94)90941-5
Publication status	Published - Sept 26 1994
Externally published	Yes

All Science Journal Classification (ASJC) codes

Neuroscience(all)

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10.1016/0304-3940(94)90941-5

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title = "Modulatory effect of plasminogen on NMDA-induced increase in intracellular free calcium concentration in rat cultured hippocampal neurons",

abstract = "The effect of plasminogen on the intracellular Ca2+ concentration ([Ca]i) was examined in cultured rat hippocampal neurons. Plasminogen (100 nM) did not increase [Ca]i in the presence of tetrodotoxin (TTX, 3 μM) but increased [Ca]i in the absence of TTX. In all the cells which responded to plasminogen (100 nM), NMDA (5 μM) also increased [Ca]i in the presence of TTX. Furthermore, plasminogen (100 nM) enhanced the NMDA-evoked [Ca]i increase, and the potentiation by plasminogen was blocked by an NMDA receptor blocker, 2-amino-phosphonovalerate (APV). These data suggest that plasminogen enhances glutamate-evoked [Ca]i increase through modulation of NMDA receptor in hippocampal neurons.",

author = "Kazuhide Inoue and Schuichi Koizumi and Kazuyuki Nakajima and Makoto Hamanoue and Shinichi Kohsaka",

year = "1994",

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language = "English",

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journal = "Neuroscience Letters",

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T1 - Modulatory effect of plasminogen on NMDA-induced increase in intracellular free calcium concentration in rat cultured hippocampal neurons

AU - Inoue, Kazuhide

AU - Koizumi, Schuichi

AU - Nakajima, Kazuyuki

AU - Hamanoue, Makoto

AU - Kohsaka, Shinichi

PY - 1994/9/26

Y1 - 1994/9/26

N2 - The effect of plasminogen on the intracellular Ca2+ concentration ([Ca]i) was examined in cultured rat hippocampal neurons. Plasminogen (100 nM) did not increase [Ca]i in the presence of tetrodotoxin (TTX, 3 μM) but increased [Ca]i in the absence of TTX. In all the cells which responded to plasminogen (100 nM), NMDA (5 μM) also increased [Ca]i in the presence of TTX. Furthermore, plasminogen (100 nM) enhanced the NMDA-evoked [Ca]i increase, and the potentiation by plasminogen was blocked by an NMDA receptor blocker, 2-amino-phosphonovalerate (APV). These data suggest that plasminogen enhances glutamate-evoked [Ca]i increase through modulation of NMDA receptor in hippocampal neurons.

AB - The effect of plasminogen on the intracellular Ca2+ concentration ([Ca]i) was examined in cultured rat hippocampal neurons. Plasminogen (100 nM) did not increase [Ca]i in the presence of tetrodotoxin (TTX, 3 μM) but increased [Ca]i in the absence of TTX. In all the cells which responded to plasminogen (100 nM), NMDA (5 μM) also increased [Ca]i in the presence of TTX. Furthermore, plasminogen (100 nM) enhanced the NMDA-evoked [Ca]i increase, and the potentiation by plasminogen was blocked by an NMDA receptor blocker, 2-amino-phosphonovalerate (APV). These data suggest that plasminogen enhances glutamate-evoked [Ca]i increase through modulation of NMDA receptor in hippocampal neurons.

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Modulatory effect of plasminogen on NMDA-induced increase in intracellular free calcium concentration in rat cultured hippocampal neurons

Abstract

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