Modulation of inwardly rectifying potassium channels in cultured bovine pulmonary artery endothelial cells

M. Kamouchi, K. Van Den Bremt, J. Eggermont, G. Droogmans, B. Nilius

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49 Citations (Scopus)


1. We have used the patch-clamp technique to study modulation of the inwardly rectifying K+ current (I(K(IR))) in cultured bovine pulmonary artery endothelial cells (CPAE cells). In whole cell mode, I(K(IR)) was defined as the Ba2+-sensitive current. In single channel recordings, we observed a strongly inwardly rectifying and (K+)-selective channel with a conductance of 31 ± 3 PS. 2. Reverse transcriptase-polymerase chain reaction (RT-PCR) analysis and functional data suggest that the endothelial IRK is most probably Kir2.1. 3. Intracellular ATP is required to prevent run-down of IRK in whole-cell mode. Single channel activity disappeared in inside-out patches exposed to ATP-free solution and in cell-attached patches on cells exposed to metabolic inhibition (KCN, 2-deoxyglucose). 4. The non-hydrolysable ATP analogues, ATPγS and adenylyl imidodiphosphate (AMP-PNP), did not prevent run-down. Run-down did not occur in the presence of okadaic acid, a phosphatase inhibitor, but was enhanced in the presence of protamine, an activator of phosphatase 2A (PP2A). 5. GTPγS and AIF4- inhibited IRK, also in the presence of ATP. GTPβS antagonized the GTPγS effect. Pretreatment of the cells with PTX did not affect the GTPγS-induced inhibition. Okadaic acid, however, slowed this inhibition. 6. Neither activation of protein kinase A (PKA) nor activation of protein kinase C (PKC) affected IRK. Additionally, neither cytochalasin B nor a high concentration of intracellular Ca2+ affected the time course of IRK run-down. We conclude that run-down of IRK is probably due to dephosphorylation by PP2A. Activation of a PTX-insensitive G protein inhibits this current by a mechanism that is neither mediated via the PKA and PKC pathways nor by intracellular Ca2+, but supposedly by a G protein-dependent activation of a phosphatase.

Original languageEnglish
Pages (from-to)545-556
Number of pages12
JournalJournal of Physiology
Issue number3
Publication statusPublished - Nov 1 1997
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Physiology


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