Modification of porous protein crystals in development of biohybrid materials

Tomomi Koshiyama, Naomi Kawaba, Tatsuo Hikage, Masanobu Shirai, Yuki Miura, Cheng Yuan Huang, Koichiro Tanaka, Yoshihito Watanabe, Takafumi Ueno

Research output: Contribution to journalArticlepeer-review

33 Citations (Scopus)


Protein assemblies have attracted increasing attention for construction of biohybrid materials. Protein crystals can also be regarded as solid protein assemblies. The present work demonstrates that protein crystals can be employed as porous biomaterials by site-specific modifications of the crystals of recombinant sperm whale myoglobin mutants. The myoglobin crystals of space group P6 provide hexagonal pores consisting of the building blocks of six Mb molecules, which form a pore with a diameter of 40 Å. On the basis of the lattice structure of the Mb crystals, we have selected appropriate residues located on the surface of the pores for replacement with cysteine. This enables modification of the pore surface via coupling with maleimide derivatives. We have succeeded in crystallizing the modified Mb mutants, retaining the P6 lattice, and consistently aligning nanosized functional molecules such as fluorescein, eosin, and Ru(bpy)3 into the hexagonal pores of the Mb crystals. Our strategy for site-specific modification of protein crystal pores is applicable to various protein crystals with porous structures. We believe that modified porous protein crystals will provide attractive candidates for novel solid materials in nanotechnology applications.

Original languageEnglish
Pages (from-to)264-269
Number of pages6
JournalBioconjugate Chemistry
Issue number2
Publication statusPublished - Feb 17 2010
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Biomedical Engineering
  • Pharmacology
  • Pharmaceutical Science
  • Organic Chemistry


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