TY - JOUR
T1 - Modeling of early neural development in vitro by direct neurosphere formation culture of chimpanzee induced pluripotent stem cells
AU - Kitajima, Ryunosuke
AU - Nakai, Risako
AU - Imamura, Takuya
AU - Kameda, Tomonori
AU - Kozuka, Daiki
AU - Hirai, Hirohisa
AU - Ito, Haruka
AU - Imai, Hiroo
AU - Imamura, Masanori
N1 - Funding Information:
We thank the staff at Kumamoto Sanctuary, Tama Zoological Park, Beppu Cable Rakutenchi, Primate Research Institute, and the Great Ape Information Network (GAIN) for chimpanzee materials. We also thank Zachary Yu-Ching Lin, Yohei Bamba, Yohei Okada, Hideyuki Okano, Kota Kuroki, Sawako Okada, Takumi Kato, Yuriko Hirai, and Kaori Yasutake for technical assistance and other support. We thank Rachel James, Ph.D. from Edanz Group (www.edanzediting.com/ac) for editing a draft of this manuscript. This work was supported by grants from the Japan Society for the Promotion of Science (JSPS); Leave a Nest Grants (Life Technologies Japan Award, On-chip Biotechnologies Award, SCREEN Holdings Award, L-RAD Award); Interuniversity Bio-Backup Project for Basic Biology; Brain Sciences Project of the Center for Novel Science Initiatives (CNTI), National Institutes of Natural Sciences (NINS); Aichi Cancer Research Foundation; Takeda Science Foundation; Kyoto University Foundation; Hori Science and Arts Foundation; Cooperation Research Program of the Primate Research Institute, Kyoto University; Extramural Collaborative Research Grant of Cancer Research Institute, Kanazawa University; and the Cooperative Study Program of National Institute for Physiological Sciences. R.N. was supported by the DAIKO Foundation and the Kyoto University Graduate School of Science Fund.
Funding Information:
We thank the staff at Kumamoto Sanctuary, Tama Zoological Park, Beppu Cable Rakutenchi, Primate Research Institute, and the Great Ape Information Network (GAIN) for chimpanzee materials. We also thank Zachary Yu-Ching Lin, Yohei Bamba, Yohei Okada, Hideyuki Okano, Kota Kuroki, Sawako Okada, Takumi Kato, Yuriko Hirai, and Kaori Yasutake for technical assistance and other support. We thank Rachel James, Ph.D., from Edanz Group ( www.edanzediting.com/ac ) for editing a draft of this manuscript. This work was supported by grants from the Japan Society for the Promotion of Science ( JSPS ); Leave a Nest Grants (Life Technologies Japan Award, On-chip Biotechnologies Award, SCREEN Holdings Award, L-RAD Award); Interuniversity Bio-Backup Project for Basic Biology; Brain Sciences Project of the Center for Novel Science Initiatives ( CNTI ), National Institutes of Natural Sciences ( NINS ); Aichi Cancer Research Foundation ; Takeda Science Foundation ; Kyoto University Foundation ; Hori Science and Arts Foundation ; Cooperation Research Program of the Primate Research Institute , Kyoto University ; Extramural Collaborative Research Grant of Cancer Research Institute , Kanazawa University ; and the Cooperative Study Program of National Institute for Physiological Sciences . R.N. was supported by the DAIKO Foundation and the Kyoto University Graduate School of Science Fund.
Publisher Copyright:
© 2020 The Author(s)
PY - 2020/4
Y1 - 2020/4
N2 - Evolutionary developmental biology of our closest living relative, the chimpanzee (Pan troglodytes), is essential for understanding the origin of human traits. However, it is difficult to access developmental events in the chimpanzee in vivo because of technical and ethical restrictions. Induced pluripotent stem cells (iPSCs) offer an alternative in vitro model system to investigate developmental events by overcoming the limitations of in vivo study. Here, we generated chimpanzee iPSCs from adult skin fibroblasts and reconstructed early neural development using in vitro differentiation culture conditions. Chimpanzee iPSCs were established using straightforward methods, namely, lipofection of plasmid vectors carrying human reprogramming factors, combined with maintenance in a comprehensive feeder-free culture. Ultimately, direct neurosphere formation culture induced rapid and efficient differentiation of neural stem cells from chimpanzee iPSCs. Time course analysis of neurosphere formation demonstrated ontogenetic changes in gene expression profiles and developmental potency along an early neural development path from epiblasts to radial glia. Our iPSC culture system is a potent tool for investigating the molecular and cellular foundation underlying chimpanzee early neural development and better understanding of human brain evolution.
AB - Evolutionary developmental biology of our closest living relative, the chimpanzee (Pan troglodytes), is essential for understanding the origin of human traits. However, it is difficult to access developmental events in the chimpanzee in vivo because of technical and ethical restrictions. Induced pluripotent stem cells (iPSCs) offer an alternative in vitro model system to investigate developmental events by overcoming the limitations of in vivo study. Here, we generated chimpanzee iPSCs from adult skin fibroblasts and reconstructed early neural development using in vitro differentiation culture conditions. Chimpanzee iPSCs were established using straightforward methods, namely, lipofection of plasmid vectors carrying human reprogramming factors, combined with maintenance in a comprehensive feeder-free culture. Ultimately, direct neurosphere formation culture induced rapid and efficient differentiation of neural stem cells from chimpanzee iPSCs. Time course analysis of neurosphere formation demonstrated ontogenetic changes in gene expression profiles and developmental potency along an early neural development path from epiblasts to radial glia. Our iPSC culture system is a potent tool for investigating the molecular and cellular foundation underlying chimpanzee early neural development and better understanding of human brain evolution.
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U2 - 10.1016/j.scr.2020.101749
DO - 10.1016/j.scr.2020.101749
M3 - Article
C2 - 32151953
AN - SCOPUS:85080987293
SN - 1873-5061
VL - 44
JO - Stem Cell Research
JF - Stem Cell Research
M1 - 101749
ER -