Minimising invasiveness in diagnostics: developing a rapid urine-based monoclonal antibody dipstick test for malaria

Uri S. Markakpo; Kwabena M. Bosompem; Mawuli Dzodzomenyo; Anthony Danso-Appiah; Edward E. Essuman; William K. Anyan; Mitsuko Suzuki; Judith K. Stephens; Isaac Anim-Baidoo; Richard H. Asmah; Michael F. Ofori; Parnor Madjitey; Jonas B. Danquah; Naa Adjeley Frempong; Kofi D. Kwofie; Michael Amoa-Bosompem; David Sullivan; Julius N. Fobil; Isabella A. Quakyi

doi:10.1111/tmi.12744

Minimising invasiveness in diagnostics: developing a rapid urine-based monoclonal antibody dipstick test for malaria

Uri S. Markakpo, Kwabena M. Bosompem, Mawuli Dzodzomenyo, Anthony Danso-Appiah, Edward E. Essuman, William K. Anyan, Mitsuko Suzuki, Judith K. Stephens, Isaac Anim-Baidoo, Richard H. Asmah, Michael F. Ofori, Parnor Madjitey, Jonas B. Danquah, Naa Adjeley Frempong, Kofi D. Kwofie, Michael Amoa-Bosompem, David Sullivan, Julius N. Fobil, Isabella A. Quakyi

Research output: Contribution to journal › Article › peer-review

3 Citations (Scopus)

Abstract

Objective: To generate monoclonal antibodies (MAbs) for developing a rapid malaria diagnostic urine-based assay (RUBDA), using Plasmodium-infected human urinary antigens. Methods: Plasmodium-infected human urinary (PAgHU) and cultured parasite (CPfAg) antigens were used to generate mouse MAbs. The reactivity and accuracy of the MAbs produced were then evaluated using microplate ELISA, SDS-PAGE, Western blotting assay, microscopy and immunochromatographic tests. Results: Ninety-six MAb clones were generated, of which 68.8% reacted to both PAgHU and CPfAg, 31.3% reacted to PAgHU only, and none reacted to CPfAg only. One promising MAb (UCP4W7) reacted in WBA, to both PAgHU and CPfAg, but not to Plasmodium-negative human urine and blood, Schistosoma haematobium and S. mansoni antigens nor measles and poliomyelitis vaccines. Conclusion: MAb UCP4W7 seems promising for diagnosing Plasmodium infection. Urine is a reliable biomarker source for developing non-invasive malaria diagnostic tests. SDS-PAGE and MAb-based WBA appear explorable in assays for detecting different levels of Plasmodium parasitaemia.

Original language	English
Pages (from-to)	1263-1271
Number of pages	9
Journal	Tropical Medicine and International Health
Volume	21
Issue number	10
DOIs	https://doi.org/10.1111/tmi.12744
Publication status	Published - Oct 1 2016
Externally published	Yes

All Science Journal Classification (ASJC) codes

Parasitology
Public Health, Environmental and Occupational Health
Infectious Diseases

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1111/tmi.12744

Cite this

Markakpo, U. S., Bosompem, K. M., Dzodzomenyo, M., Danso-Appiah, A., Essuman, E. E., Anyan, W. K., Suzuki, M., Stephens, J. K., Anim-Baidoo, I., Asmah, R. H., Ofori, M. F., Madjitey, P., Danquah, J. B., Frempong, N. A., Kwofie, K. D., Amoa-Bosompem, M., Sullivan, D., Fobil, J. N., & Quakyi, I. A. (2016). Minimising invasiveness in diagnostics: developing a rapid urine-based monoclonal antibody dipstick test for malaria. Tropical Medicine and International Health, 21(10), 1263-1271. https://doi.org/10.1111/tmi.12744

Markakpo, US, Bosompem, KM, Dzodzomenyo, M, Danso-Appiah, A, Essuman, EE, Anyan, WK, Suzuki, M, Stephens, JK, Anim-Baidoo, I, Asmah, RH, Ofori, MF, Madjitey, P, Danquah, JB, Frempong, NA, Kwofie, KD, Amoa-Bosompem, M, Sullivan, D, Fobil, JN & Quakyi, IA 2016, 'Minimising invasiveness in diagnostics: developing a rapid urine-based monoclonal antibody dipstick test for malaria', Tropical Medicine and International Health, vol. 21, no. 10, pp. 1263-1271. https://doi.org/10.1111/tmi.12744

@article{914ab7e1259e49fb93873917b301faee,

title = "Minimising invasiveness in diagnostics: developing a rapid urine-based monoclonal antibody dipstick test for malaria",

abstract = "Objective: To generate monoclonal antibodies (MAbs) for developing a rapid malaria diagnostic urine-based assay (RUBDA), using Plasmodium-infected human urinary antigens. Methods: Plasmodium-infected human urinary (PAgHU) and cultured parasite (CPfAg) antigens were used to generate mouse MAbs. The reactivity and accuracy of the MAbs produced were then evaluated using microplate ELISA, SDS-PAGE, Western blotting assay, microscopy and immunochromatographic tests. Results: Ninety-six MAb clones were generated, of which 68.8% reacted to both PAgHU and CPfAg, 31.3% reacted to PAgHU only, and none reacted to CPfAg only. One promising MAb (UCP4W7) reacted in WBA, to both PAgHU and CPfAg, but not to Plasmodium-negative human urine and blood, Schistosoma haematobium and S. mansoni antigens nor measles and poliomyelitis vaccines. Conclusion: MAb UCP4W7 seems promising for diagnosing Plasmodium infection. Urine is a reliable biomarker source for developing non-invasive malaria diagnostic tests. SDS-PAGE and MAb-based WBA appear explorable in assays for detecting different levels of Plasmodium parasitaemia.",

author = "Markakpo, {Uri S.} and Bosompem, {Kwabena M.} and Mawuli Dzodzomenyo and Anthony Danso-Appiah and Essuman, {Edward E.} and Anyan, {William K.} and Mitsuko Suzuki and Stephens, {Judith K.} and Isaac Anim-Baidoo and Asmah, {Richard H.} and Ofori, {Michael F.} and Parnor Madjitey and Danquah, {Jonas B.} and Frempong, {Naa Adjeley} and Kwofie, {Kofi D.} and Michael Amoa-Bosompem and David Sullivan and Fobil, {Julius N.} and Quakyi, {Isabella A.}",

note = "Publisher Copyright: {\textcopyright} 2016 John Wiley & Sons Ltd",

year = "2016",

month = oct,

day = "1",

doi = "10.1111/tmi.12744",

language = "English",

volume = "21",

pages = "1263--1271",

journal = "Tropical Medicine and International Health",

issn = "1360-2276",

publisher = "Wiley-Blackwell",

number = "10",

}

TY - JOUR

T1 - Minimising invasiveness in diagnostics

T2 - developing a rapid urine-based monoclonal antibody dipstick test for malaria

AU - Markakpo, Uri S.

AU - Bosompem, Kwabena M.

AU - Dzodzomenyo, Mawuli

AU - Danso-Appiah, Anthony

AU - Essuman, Edward E.

AU - Anyan, William K.

AU - Suzuki, Mitsuko

AU - Stephens, Judith K.

AU - Anim-Baidoo, Isaac

AU - Asmah, Richard H.

AU - Ofori, Michael F.

AU - Madjitey, Parnor

AU - Danquah, Jonas B.

AU - Frempong, Naa Adjeley

AU - Kwofie, Kofi D.

AU - Amoa-Bosompem, Michael

AU - Sullivan, David

AU - Fobil, Julius N.

AU - Quakyi, Isabella A.

PY - 2016/10/1

Y1 - 2016/10/1

N2 - Objective: To generate monoclonal antibodies (MAbs) for developing a rapid malaria diagnostic urine-based assay (RUBDA), using Plasmodium-infected human urinary antigens. Methods: Plasmodium-infected human urinary (PAgHU) and cultured parasite (CPfAg) antigens were used to generate mouse MAbs. The reactivity and accuracy of the MAbs produced were then evaluated using microplate ELISA, SDS-PAGE, Western blotting assay, microscopy and immunochromatographic tests. Results: Ninety-six MAb clones were generated, of which 68.8% reacted to both PAgHU and CPfAg, 31.3% reacted to PAgHU only, and none reacted to CPfAg only. One promising MAb (UCP4W7) reacted in WBA, to both PAgHU and CPfAg, but not to Plasmodium-negative human urine and blood, Schistosoma haematobium and S. mansoni antigens nor measles and poliomyelitis vaccines. Conclusion: MAb UCP4W7 seems promising for diagnosing Plasmodium infection. Urine is a reliable biomarker source for developing non-invasive malaria diagnostic tests. SDS-PAGE and MAb-based WBA appear explorable in assays for detecting different levels of Plasmodium parasitaemia.

AB - Objective: To generate monoclonal antibodies (MAbs) for developing a rapid malaria diagnostic urine-based assay (RUBDA), using Plasmodium-infected human urinary antigens. Methods: Plasmodium-infected human urinary (PAgHU) and cultured parasite (CPfAg) antigens were used to generate mouse MAbs. The reactivity and accuracy of the MAbs produced were then evaluated using microplate ELISA, SDS-PAGE, Western blotting assay, microscopy and immunochromatographic tests. Results: Ninety-six MAb clones were generated, of which 68.8% reacted to both PAgHU and CPfAg, 31.3% reacted to PAgHU only, and none reacted to CPfAg only. One promising MAb (UCP4W7) reacted in WBA, to both PAgHU and CPfAg, but not to Plasmodium-negative human urine and blood, Schistosoma haematobium and S. mansoni antigens nor measles and poliomyelitis vaccines. Conclusion: MAb UCP4W7 seems promising for diagnosing Plasmodium infection. Urine is a reliable biomarker source for developing non-invasive malaria diagnostic tests. SDS-PAGE and MAb-based WBA appear explorable in assays for detecting different levels of Plasmodium parasitaemia.

UR - http://www.scopus.com/inward/record.url?scp=84991086157&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84991086157&partnerID=8YFLogxK

U2 - 10.1111/tmi.12744

DO - 10.1111/tmi.12744

M3 - Article

C2 - 27546068

AN - SCOPUS:84991086157

SN - 1360-2276

VL - 21

SP - 1263

EP - 1271

JO - Tropical Medicine and International Health

JF - Tropical Medicine and International Health

IS - 10

ER -

Minimising invasiveness in diagnostics: developing a rapid urine-based monoclonal antibody dipstick test for malaria

Abstract

All Science Journal Classification (ASJC) codes

UN SDGs

Access to Document

Other files and links

Fingerprint

Cite this