TY - JOUR
T1 - Midkine mRNA is overexpressed in pancreatic cancer
AU - Ohhashi, Seiji
AU - Ohuchida, Kenoki
AU - Mizumoto, Kazuhiro
AU - Egami, Takuya
AU - Yu, Jun
AU - Cui, Lin
AU - Toma, Hiroki
AU - Takahata, Shunichi
AU - Nabae, Toshinaga
AU - Tanaka, Masao
N1 - Funding Information:
Acknowledgments This research work was supported in part by a Grant-in-Aid from the Ministry of Education, Culture, Sports, Science and Technology of Japan, and grants from the Fukuoka Cancer Society and Kaibara Morikazu Medical Science Promotion Foundation. We are grateful to Emiko Manabe, Makiko Masuda, and Miyuki Omori (Department of Surgery and Oncology, Kyushu University) for skillful technical assistance.
PY - 2009/4
Y1 - 2009/4
N2 - Purpose Midkine (MK) has been reported to be a possible molecular marker for the diagnosis of pancreatic cancer. We investigated the feasibility of quantitative analysis of MK mRNA by quantitative real-time RT-PCR (qRT-PCR) as a promising tool for the diagnosis of pancreatic cancer. Results We found that pancreatic cancer tissues expressed significantly higher levels of MK mRNA than intraductal pancreatic mucinous neoplasm (IPMN) and non-neoplastic pancreatic tissues (P < 0.05); in contrast, we did not find any differences in MK mRNA expression between IPMN and non-neoplastic pancreatic tissues. Additionally, we observed that poorly differentiated carcinoma samples expressed higher levels of MK mRNA than well-differentiated carcinoma samples, although a significant difference was not observed. Conclusions The present data suggests that quantitative analysis of MK mRNA provides an objective and sensitive evaluation and may be a promising modality for the diagnosis of pancreatic cancer and the prediction of its prognosis.
AB - Purpose Midkine (MK) has been reported to be a possible molecular marker for the diagnosis of pancreatic cancer. We investigated the feasibility of quantitative analysis of MK mRNA by quantitative real-time RT-PCR (qRT-PCR) as a promising tool for the diagnosis of pancreatic cancer. Results We found that pancreatic cancer tissues expressed significantly higher levels of MK mRNA than intraductal pancreatic mucinous neoplasm (IPMN) and non-neoplastic pancreatic tissues (P < 0.05); in contrast, we did not find any differences in MK mRNA expression between IPMN and non-neoplastic pancreatic tissues. Additionally, we observed that poorly differentiated carcinoma samples expressed higher levels of MK mRNA than well-differentiated carcinoma samples, although a significant difference was not observed. Conclusions The present data suggests that quantitative analysis of MK mRNA provides an objective and sensitive evaluation and may be a promising modality for the diagnosis of pancreatic cancer and the prediction of its prognosis.
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U2 - 10.1007/s10620-008-0434-4
DO - 10.1007/s10620-008-0434-4
M3 - Article
C2 - 18712601
AN - SCOPUS:63249116871
SN - 0163-2116
VL - 54
SP - 811
EP - 815
JO - Digestive Diseases and Sciences
JF - Digestive Diseases and Sciences
IS - 4
ER -
