MicroRNAs miR-4535 and miR-1915-5p in amniotic fluid as predictive biomarkers for chorioamnionitis

Chihiro Kiyoshima, Naoto Shirasu, Daichi Urushiyama, Satoshi Fukagawa, Toyofumi Hirakawa, Kenichi Yoshikawa, Daisuke Izuchi, Kohei Miyata, Masamitsu Kurakazu, Fusanori Yotsumoto, Kenji Hiromatsu, Makoto Nomiyama, Ohta Eiji, Shinichi Hirose, Yoshitoshi Ogura, Tetsuya Hayashi, Kenichiro Hata, Kazuki Nabeshima, Shin'ichiro Yasunaga, Shingo Miyamoto

Research output: Contribution to journalArticlepeer-review

3 Citations (Scopus)


Background: This study was performed to investigate the clinical significance of miR-4535 and miR-1915-5p in severe chorioamnionitis. Materials and methods: Amniotic fluid samples from 37 patients with severe chorioamnionitis were subjected to miRNA array analysis and ddPCR™. Diagnostic values were assessed using the receiver operating characteristic curve. The patients were separated into three groups according to Blanc's criteria. Results: The expression of miR-4535 and miR-1915-5p was significantly correlated with the copy number of 16S rDNA, had extremely high diagnostic accuracy for severe chorioamnionitis, and was linked to maternal and fetal inflammation. Conclusion: MiR-4535 and miR-1915-5p serve as promising biomarkers for the diagnosis of severe chorioamnionitis. Chorioamnionitis and fetal inflammatory response syndrome, which is linked to chorioamnionitis, are considered serious diseases in perinatal care. In this study, miR-4535 and miR-1915-5p are recognized as promising biomarkers for the diagnosis of chorioamnionitis before delivery. In particular, the increased expression of miR-4535 in amniotic fluid is expected to be regarded as a positive indicator for fetal inflammatory response syndrome, and the elevated expression of miR-4535 in serum is also considered to predictively diagnose intrauterine infection in pregnancy. Our results highlight that further studies should explore the underlying clinical significance of miR-4535 and miR-1915-5p.

Original languageEnglish
Article numberFSO686
JournalFuture Science OA
Issue number5
Publication statusPublished - Jun 2021

All Science Journal Classification (ASJC) codes

  • Biotechnology


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