Microprocessing of liquid plugs for bio/chemical analyses

Fumihiro Sassa, Junji Fukuda, Hiroaki Suzuki

Research output: Contribution to journalArticlepeer-review

40 Citations (Scopus)


A microfluidic device and operation to handle liquid plugs for biochemical analyses were developed for efficient handling of plugs of many solutions. A major part of the device was a T-junction consisting of a main flow channel and a handling flow channel. Unit operations including attachment of plugs, division of a plug, sorting of plugs, and formation of plugs of various lengths enabled controlled sequential reactions in a microflow channel. Rapid mixing could easily be achieved by moving a plug formed by merging two plugs back and forth. The device could be used for efficient characterization of performance in bio/chemical sensing. In experiments using L-glutamate oxidase, plugs containing an enzyme or a substrate were formed, mixed sequentially, and the intensity of fluorescence from plugs of different concentrations of L-glutamate or pHs could be measured simultaneously. Cross-contamination of plugs by neighboring plugs poses a problem in using the same flow channel repeatedly. However, the influence could be minimized by using a cleansing plug placed between them in a sufficiently hydrophobic flow channel and by processing the plugs at a low velocity. The device can be a critical component for microprocessing in various bio/chemical analyses.

Original languageEnglish
Pages (from-to)6206-6213
Number of pages8
JournalAnalytical Chemistry
Issue number16
Publication statusPublished - Aug 15 2008
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry


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