TY - GEN
T1 - Microheater-nanowires device for detection of cell-free circulating methylated DNA
AU - Takahashi, Hiromi
AU - Yasui, Takao
AU - Shinjo, Keiko
AU - Kaji, Noritada
AU - Okamoto, Akimitsu
AU - Baba, Yoshinobu
PY - 2018/1/1
Y1 - 2018/1/1
N2 - Detecting of cell-free circulating methylated DNA could be a promising method for early diagnosis of cancer. Here we have developed the microheater - zinc oxide nanowires (ZnO-NWs) structures embedded in the microchannel device for capturing DNA via electrostatic interaction. We found that 60% of DNA could be captured on ZnO-NWs. Additionally, the microheater, which constantly enables to generate heat, can denature double stranded DNA (dsDNA) to single stranded DNA (ssDNA). Finally, ICON Probe, which is an artificial ssDNA probe for DNA methylation, was introduced inside a device to bind to ssDNA for DNA methylation detection.
AB - Detecting of cell-free circulating methylated DNA could be a promising method for early diagnosis of cancer. Here we have developed the microheater - zinc oxide nanowires (ZnO-NWs) structures embedded in the microchannel device for capturing DNA via electrostatic interaction. We found that 60% of DNA could be captured on ZnO-NWs. Additionally, the microheater, which constantly enables to generate heat, can denature double stranded DNA (dsDNA) to single stranded DNA (ssDNA). Finally, ICON Probe, which is an artificial ssDNA probe for DNA methylation, was introduced inside a device to bind to ssDNA for DNA methylation detection.
UR - http://www.scopus.com/inward/record.url?scp=85079785919&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85079785919&partnerID=8YFLogxK
M3 - Conference contribution
T3 - 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018
SP - 1886
EP - 1888
BT - 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018
PB - Chemical and Biological Microsystems Society
T2 - 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018
Y2 - 11 November 2018 through 15 November 2018
ER -
