TY - GEN
T1 - Microfluidic visualization of encounter complex in enzymatic digestion of single DNA molecule by dual molecular tagging
AU - Onoshima, D.
AU - Kaji, N.
AU - Tokeshi, M.
AU - Baba, Y.
PY - 2011
Y1 - 2011
N2 - We combined molecular tagging with microfluidics to study kinematics in a single DNA molecular digestion. Moving tags of both DNA ends revealed initial breakup of a single DNA molecule into fragments induced by limited enzymatic digestion and subsequent rapid formation of transient structure in encounter complex. The method enables us to analyze rapid dissociation of weakly bound complexes derived from intramolecular transfer.
AB - We combined molecular tagging with microfluidics to study kinematics in a single DNA molecular digestion. Moving tags of both DNA ends revealed initial breakup of a single DNA molecule into fragments induced by limited enzymatic digestion and subsequent rapid formation of transient structure in encounter complex. The method enables us to analyze rapid dissociation of weakly bound complexes derived from intramolecular transfer.
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UR - http://www.scopus.com/inward/citedby.url?scp=84883754075&partnerID=8YFLogxK
M3 - Conference contribution
AN - SCOPUS:84883754075
SN - 9781618395955
T3 - 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
SP - 2061
EP - 2063
BT - 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
T2 - 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
Y2 - 2 October 2011 through 6 October 2011
ER -