TY - GEN
T1 - Microfluidic sensor for the detection of DNA or protein by hybridization-based fluorescence enhancement or immunoassay-based fluorescence quenching
AU - Wang, J.
AU - Aki, M.
AU - Onoshima, D.
AU - Arinaga, K.
AU - Kaji, N.
AU - Tokeshi, M.
AU - Fujita, S.
AU - Yokoyama, N.
AU - Baba, Y.
PY - 2010
Y1 - 2010
N2 - We have developed a novel optical DNA/protein sensor which consists of single-stranded (ss) DNA-Cy3 probes on gold surface and one line-shape polydimethylsiloxane (PDMS) microfluidic channel. This novel ssDNA-Cy3 probes, which does not require hairpin-like stem-loop conformation, show similar advantages as traditional molecular beacon technique in this microfluidic sensor. This chip-based optical sensor showed strong fluorescence enhancement by the binding of cDNA or cDNA-biotin. After introduction of cDNA-biotin, streptavidin can be determined by fluorescence quenching. This sensor showed strong affinity and high sensitivity toward streptavidin, the minimum detectable concentration for streptavidin was 1 pM, equating to an absolute detection limit of 50 amol in this sensor.
AB - We have developed a novel optical DNA/protein sensor which consists of single-stranded (ss) DNA-Cy3 probes on gold surface and one line-shape polydimethylsiloxane (PDMS) microfluidic channel. This novel ssDNA-Cy3 probes, which does not require hairpin-like stem-loop conformation, show similar advantages as traditional molecular beacon technique in this microfluidic sensor. This chip-based optical sensor showed strong fluorescence enhancement by the binding of cDNA or cDNA-biotin. After introduction of cDNA-biotin, streptavidin can be determined by fluorescence quenching. This sensor showed strong affinity and high sensitivity toward streptavidin, the minimum detectable concentration for streptavidin was 1 pM, equating to an absolute detection limit of 50 amol in this sensor.
UR - http://www.scopus.com/inward/record.url?scp=84884306304&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84884306304&partnerID=8YFLogxK
M3 - Conference contribution
AN - SCOPUS:84884306304
SN - 9781618390622
T3 - 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010
SP - 1514
EP - 1516
BT - 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010
T2 - 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010
Y2 - 3 October 2010 through 7 October 2010
ER -