TY - GEN
T1 - MICROFLUIDIC METHODOLOGIES FOR PRODUCTION OF SMALL-SIZED VARIOUS ARTIFICIAL EXOSOMES
AU - Kimura, Niko
AU - Nomiyama, Takuma
AU - Naito, Toyohiro
AU - Maeki, Masatoshi
AU - Tokeshi, Manabu
AU - Kaji, Noritada
N1 - Funding Information:
This work was supported by Fostering Joint International Research (B) (19KK0140).
Publisher Copyright:
© 2021 MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences. All rights reserved.
PY - 2021
Y1 - 2021
N2 - This paper describes microfluidic methodologies for production of sub-100 nm sized various types of artificial exosomes by separately injecting of phospholipids dissolved ethanol solution. The separated injection method allowed production of smaller-sized artificial exosomes with high encapsulation efficiency of small interfering RNA (siRNA) regardless of natural exosome-like lipid systems than the conventional combined injection method. The prepared artificial exosomes were taken into cells depending on the average sizes, lipid systems, and cell types. This microfluidic method is expected to contribute to exosomal research and development of exosomal nanomedicines.
AB - This paper describes microfluidic methodologies for production of sub-100 nm sized various types of artificial exosomes by separately injecting of phospholipids dissolved ethanol solution. The separated injection method allowed production of smaller-sized artificial exosomes with high encapsulation efficiency of small interfering RNA (siRNA) regardless of natural exosome-like lipid systems than the conventional combined injection method. The prepared artificial exosomes were taken into cells depending on the average sizes, lipid systems, and cell types. This microfluidic method is expected to contribute to exosomal research and development of exosomal nanomedicines.
UR - http://www.scopus.com/inward/record.url?scp=85136914764&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85136914764&partnerID=8YFLogxK
M3 - Conference contribution
AN - SCOPUS:85136914764
T3 - MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences
SP - 355
EP - 356
BT - MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences
PB - Chemical and Biological Microsystems Society
T2 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2021
Y2 - 10 October 2021 through 14 October 2021
ER -