Microfluidic electrochemiluminescence (ECL) integrated flow cell for portable fluorescence detection

Miho Tsuwaki; Jun Mizuno; Takashi Kasahara; Tomohiko Edura; Eri Kunisawa; Ryoichi Ishimatsu; Shigeyuki Matsunami; Toshihiko Imato; Chihaya Adachi; Shuichi Shoji

doi:10.1109/MEMSYS.2014.6765585

Microfluidic electrochemiluminescence (ECL) integrated flow cell for portable fluorescence detection

Miho Tsuwaki, Jun Mizuno, Takashi Kasahara, Tomohiko Edura, Eri Kunisawa, Ryoichi Ishimatsu, Shigeyuki Matsunami, Toshihiko Imato, Chihaya Adachi, Shuichi Shoji

Applied Chemistry

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

6 Citations (Scopus)

Abstract

We propose a portable electrochemiluminescence (ECL)-induced fluorescence chip which consists of flow channels for fluorescence sample and multi-color emitting ECL excitation source. A prototype ECL-induced fluorescence chip was fabricated by conventional photolithography and bonding technique. Device performance was evaluated using ECL of rubrene as excitation source and resorufin as fluorescent dye. Fluorescence of 500 μM resorufin (600 nm) was successfully detected using 10 mM rubrene solution (560 nm) under the applied voltage of 4 V. The proposed principle is applicable for portable and on-demand multi fluorescence detection device using its freedom of choice for combination of the ECL light source.

Original language	English
Title of host publication	MEMS 2014 - 27th IEEE International Conference on Micro Electro Mechanical Systems
Publisher	Institute of Electrical and Electronics Engineers Inc.
Pages	108-111
Number of pages	4
ISBN (Print)	9781479935086
DOIs	https://doi.org/10.1109/MEMSYS.2014.6765585
Publication status	Published - 2014
Event	27th IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2014 - San Francisco, CA, United States Duration: Jan 26 2014 → Jan 30 2014

Other

Other	27th IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2014
Country/Territory	United States
City	San Francisco, CA
Period	1/26/14 → 1/30/14

All Science Journal Classification (ASJC) codes

Electronic, Optical and Magnetic Materials
Condensed Matter Physics
Mechanical Engineering
Electrical and Electronic Engineering

Access to Document

10.1109/MEMSYS.2014.6765585

Cite this

Tsuwaki, M., Mizuno, J., Kasahara, T., Edura, T., Kunisawa, E., Ishimatsu, R., Matsunami, S., Imato, T., Adachi, C., & Shoji, S. (2014). Microfluidic electrochemiluminescence (ECL) integrated flow cell for portable fluorescence detection. In MEMS 2014 - 27th IEEE International Conference on Micro Electro Mechanical Systems (pp. 108-111). Article 6765585 Institute of Electrical and Electronics Engineers Inc.. https://doi.org/10.1109/MEMSYS.2014.6765585

Microfluidic electrochemiluminescence (ECL) integrated flow cell for portable fluorescence detection. / Tsuwaki, Miho; Mizuno, Jun; Kasahara, Takashi et al.
MEMS 2014 - 27th IEEE International Conference on Micro Electro Mechanical Systems. Institute of Electrical and Electronics Engineers Inc., 2014. p. 108-111 6765585.

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Tsuwaki, M, Mizuno, J, Kasahara, T, Edura, T, Kunisawa, E, Ishimatsu, R, Matsunami, S, Imato, T, Adachi, C & Shoji, S 2014, Microfluidic electrochemiluminescence (ECL) integrated flow cell for portable fluorescence detection. in MEMS 2014 - 27th IEEE International Conference on Micro Electro Mechanical Systems., 6765585, Institute of Electrical and Electronics Engineers Inc., pp. 108-111, 27th IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2014, San Francisco, CA, United States, 1/26/14. https://doi.org/10.1109/MEMSYS.2014.6765585

Tsuwaki M, Mizuno J, Kasahara T, Edura T, Kunisawa E, Ishimatsu R et al. Microfluidic electrochemiluminescence (ECL) integrated flow cell for portable fluorescence detection. In MEMS 2014 - 27th IEEE International Conference on Micro Electro Mechanical Systems. Institute of Electrical and Electronics Engineers Inc. 2014. p. 108-111. 6765585 doi: 10.1109/MEMSYS.2014.6765585

@inproceedings{23699b6344c84a90810321a2c847e2b6,

title = "Microfluidic electrochemiluminescence (ECL) integrated flow cell for portable fluorescence detection",

abstract = "We propose a portable electrochemiluminescence (ECL)-induced fluorescence chip which consists of flow channels for fluorescence sample and multi-color emitting ECL excitation source. A prototype ECL-induced fluorescence chip was fabricated by conventional photolithography and bonding technique. Device performance was evaluated using ECL of rubrene as excitation source and resorufin as fluorescent dye. Fluorescence of 500 μM resorufin (600 nm) was successfully detected using 10 mM rubrene solution (560 nm) under the applied voltage of 4 V. The proposed principle is applicable for portable and on-demand multi fluorescence detection device using its freedom of choice for combination of the ECL light source.",

author = "Miho Tsuwaki and Jun Mizuno and Takashi Kasahara and Tomohiko Edura and Eri Kunisawa and Ryoichi Ishimatsu and Shigeyuki Matsunami and Toshihiko Imato and Chihaya Adachi and Shuichi Shoji",

year = "2014",

doi = "10.1109/MEMSYS.2014.6765585",

language = "English",

isbn = "9781479935086",

pages = "108--111",

booktitle = "MEMS 2014 - 27th IEEE International Conference on Micro Electro Mechanical Systems",

publisher = "Institute of Electrical and Electronics Engineers Inc.",

address = "United States",

note = "27th IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2014 ; Conference date: 26-01-2014 Through 30-01-2014",

}

TY - GEN

T1 - Microfluidic electrochemiluminescence (ECL) integrated flow cell for portable fluorescence detection

AU - Tsuwaki, Miho

AU - Mizuno, Jun

AU - Kasahara, Takashi

AU - Edura, Tomohiko

AU - Kunisawa, Eri

AU - Ishimatsu, Ryoichi

AU - Matsunami, Shigeyuki

AU - Imato, Toshihiko

AU - Adachi, Chihaya

AU - Shoji, Shuichi

PY - 2014

Y1 - 2014

N2 - We propose a portable electrochemiluminescence (ECL)-induced fluorescence chip which consists of flow channels for fluorescence sample and multi-color emitting ECL excitation source. A prototype ECL-induced fluorescence chip was fabricated by conventional photolithography and bonding technique. Device performance was evaluated using ECL of rubrene as excitation source and resorufin as fluorescent dye. Fluorescence of 500 μM resorufin (600 nm) was successfully detected using 10 mM rubrene solution (560 nm) under the applied voltage of 4 V. The proposed principle is applicable for portable and on-demand multi fluorescence detection device using its freedom of choice for combination of the ECL light source.

AB - We propose a portable electrochemiluminescence (ECL)-induced fluorescence chip which consists of flow channels for fluorescence sample and multi-color emitting ECL excitation source. A prototype ECL-induced fluorescence chip was fabricated by conventional photolithography and bonding technique. Device performance was evaluated using ECL of rubrene as excitation source and resorufin as fluorescent dye. Fluorescence of 500 μM resorufin (600 nm) was successfully detected using 10 mM rubrene solution (560 nm) under the applied voltage of 4 V. The proposed principle is applicable for portable and on-demand multi fluorescence detection device using its freedom of choice for combination of the ECL light source.

UR - http://www.scopus.com/inward/record.url?scp=84898967601&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84898967601&partnerID=8YFLogxK

U2 - 10.1109/MEMSYS.2014.6765585

DO - 10.1109/MEMSYS.2014.6765585

M3 - Conference contribution

AN - SCOPUS:84898967601

SN - 9781479935086

SP - 108

EP - 111

BT - MEMS 2014 - 27th IEEE International Conference on Micro Electro Mechanical Systems

PB - Institute of Electrical and Electronics Engineers Inc.

T2 - 27th IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2014

Y2 - 26 January 2014 through 30 January 2014

ER -

Microfluidic electrochemiluminescence (ECL) integrated flow cell for portable fluorescence detection

Abstract

Other

All Science Journal Classification (ASJC) codes

Access to Document

Other files and links

Fingerprint

Cite this