TY - GEN
T1 - Microfluidic Chip for Simultaneous Loading and Injection of a Single Cell by Using Rack and Pinion Mechanisms
AU - Uning, Kevin T.
AU - Ichikawa, Keita
AU - Hirao, Akiho
AU - Michimoto, Taiga
AU - Sato, Tasuku
AU - Kume, Hiroaki
AU - Nishida, Takumi
AU - Iwakawa, Shota
AU - Yamanishi, Yoko
N1 - Funding Information:
This work was supported by JST CREST Grant No. JPMJCR19S6, JST A-STEP(AS3015134U), and AMED SENTAN (grant Number JP17hm0102049).
Publisher Copyright:
© 2020 IEEE.
PY - 2020/1
Y1 - 2020/1
N2 - We present a novel microinjection chip, which combines a pinion-rack cell loader and a 3-dimensional bubble injector probe, in order to increase the efficiency of microinjection and simplify it. The loader features a powerful interaction with magnets, capable of lifting as much as 20 grams with a 400mT strong magnet. Additionally, the bubble injector is easy to use while also being reliable in doing microinjections. Consequently, not only that the loader can work under harsh conditions, it does so while maintaining a high throughput of up to 16 gel beads (as the model of cells) per minutes. Combining this reliable loader with the easily operated injector, this chip will allow anyone with little to no training to perform microinjections.
AB - We present a novel microinjection chip, which combines a pinion-rack cell loader and a 3-dimensional bubble injector probe, in order to increase the efficiency of microinjection and simplify it. The loader features a powerful interaction with magnets, capable of lifting as much as 20 grams with a 400mT strong magnet. Additionally, the bubble injector is easy to use while also being reliable in doing microinjections. Consequently, not only that the loader can work under harsh conditions, it does so while maintaining a high throughput of up to 16 gel beads (as the model of cells) per minutes. Combining this reliable loader with the easily operated injector, this chip will allow anyone with little to no training to perform microinjections.
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U2 - 10.1109/MEMS46641.2020.9056117
DO - 10.1109/MEMS46641.2020.9056117
M3 - Conference contribution
AN - SCOPUS:85083197078
T3 - Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)
SP - 392
EP - 395
BT - 33rd IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2020
PB - Institute of Electrical and Electronics Engineers Inc.
T2 - 33rd IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2020
Y2 - 18 January 2020 through 22 January 2020
ER -