TY - GEN
T1 - Microfluidic approach for production of lipid nanoparticles-based nano medicine
AU - Maeki, M.
AU - Saito, T.
AU - Node, Y.
AU - Sato, Y.
AU - Yasui, T.
AU - Kaji, N.
AU - Ishida, A.
AU - Tani, H.
AU - Baba, Y.
AU - Harashima, H.
AU - Tokeshi, M.
N1 - Funding Information:
Part of this work was supported by the Hosokawa Powder Technology Foundation. M. Maeki acknowledges support from the Japan Society for the Promotion of Science (JSPS).
Publisher Copyright:
© 15CBMS-0001.
PY - 2015
Y1 - 2015
N2 - This paper described a simple preparation method for small-size and monodispersed lipid nanoparticles (LNPs) by using microfluidic devices. The fundamental role and importance of chaotic micromixer in the microfluidic device was demonstrated. The suitable cycle number of chaotic micromixer was confirmed for precise controlling LNPs size with narrow distribution under the any flow rate conditions. In addition, LNPs containing siRNA was synthesized for evaluation of penetration efficiency via in vivo experiment. The PEGylated LNPs containing siRNA with a diameter of 30 nm could penetrate to the mouse parenchymal liver cells rather than the LNPs with a diameter of 50 nm.
AB - This paper described a simple preparation method for small-size and monodispersed lipid nanoparticles (LNPs) by using microfluidic devices. The fundamental role and importance of chaotic micromixer in the microfluidic device was demonstrated. The suitable cycle number of chaotic micromixer was confirmed for precise controlling LNPs size with narrow distribution under the any flow rate conditions. In addition, LNPs containing siRNA was synthesized for evaluation of penetration efficiency via in vivo experiment. The PEGylated LNPs containing siRNA with a diameter of 30 nm could penetrate to the mouse parenchymal liver cells rather than the LNPs with a diameter of 50 nm.
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M3 - Conference contribution
AN - SCOPUS:84983250238
T3 - MicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences
SP - 838
EP - 840
BT - MicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences
PB - Chemical and Biological Microsystems Society
T2 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2015
Y2 - 25 October 2015 through 29 October 2015
ER -