Microchip-based homogeneous immunoassay using a cloned enzyme donor

Tomoya Tachi; Noritada Kaji; Manabu Tokeshi; Yoshinobu Baba

doi:10.2116/analsci.25.149

Microchip-based homogeneous immunoassay using a cloned enzyme donor

Tomoya Tachi, Noritada Kaji, Manabu Tokeshi, Yoshinobu Baba

Research output: Contribution to journal › Article › peer-review

12 Citations (Scopus)

Abstract

We have realized a cloned enzyme donor immunoassay (CEDIA) on a microchip in 96 s. CEDIA is a homogeneous immunoassay, based on the bacterial enzyme β-galactosidase, which was genetically engineered into two inactive fragments: an enzyme donor and an enzyme acceptor. A model analyte was theophylline, and the detectable concentration range was from 0 to 40 μg mL^-1. Our CEDIA using a microfluidic device was very simple and rapid, unlike microchipbased heterogeneous immunoassays and CEDIA on a well-type microchip.

Original language	English
Pages (from-to)	149-151
Number of pages	3
Journal	analytical sciences
Volume	25
Issue number	2
DOIs	https://doi.org/10.2116/analsci.25.149
Publication status	Published - 2009
Externally published	Yes

All Science Journal Classification (ASJC) codes

Analytical Chemistry

Access to Document

10.2116/analsci.25.149

Cite this

@article{d9a1b2098b324b5cb19a169973c9c559,

title = "Microchip-based homogeneous immunoassay using a cloned enzyme donor",

abstract = "We have realized a cloned enzyme donor immunoassay (CEDIA) on a microchip in 96 s. CEDIA is a homogeneous immunoassay, based on the bacterial enzyme β-galactosidase, which was genetically engineered into two inactive fragments: an enzyme donor and an enzyme acceptor. A model analyte was theophylline, and the detectable concentration range was from 0 to 40 μg mL-1. Our CEDIA using a microfluidic device was very simple and rapid, unlike microchipbased heterogeneous immunoassays and CEDIA on a well-type microchip.",

author = "Tomoya Tachi and Noritada Kaji and Manabu Tokeshi and Yoshinobu Baba",

year = "2009",

doi = "10.2116/analsci.25.149",

language = "English",

volume = "25",

pages = "149--151",

journal = "analytical sciences",

issn = "0910-6340",

publisher = "The Japan Society for Analytical Chemistry",

number = "2",

}

TY - JOUR

T1 - Microchip-based homogeneous immunoassay using a cloned enzyme donor

AU - Tachi, Tomoya

AU - Kaji, Noritada

AU - Tokeshi, Manabu

AU - Baba, Yoshinobu

PY - 2009

Y1 - 2009

N2 - We have realized a cloned enzyme donor immunoassay (CEDIA) on a microchip in 96 s. CEDIA is a homogeneous immunoassay, based on the bacterial enzyme β-galactosidase, which was genetically engineered into two inactive fragments: an enzyme donor and an enzyme acceptor. A model analyte was theophylline, and the detectable concentration range was from 0 to 40 μg mL-1. Our CEDIA using a microfluidic device was very simple and rapid, unlike microchipbased heterogeneous immunoassays and CEDIA on a well-type microchip.

AB - We have realized a cloned enzyme donor immunoassay (CEDIA) on a microchip in 96 s. CEDIA is a homogeneous immunoassay, based on the bacterial enzyme β-galactosidase, which was genetically engineered into two inactive fragments: an enzyme donor and an enzyme acceptor. A model analyte was theophylline, and the detectable concentration range was from 0 to 40 μg mL-1. Our CEDIA using a microfluidic device was very simple and rapid, unlike microchipbased heterogeneous immunoassays and CEDIA on a well-type microchip.

UR - http://www.scopus.com/inward/record.url?scp=63149149476&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=63149149476&partnerID=8YFLogxK

U2 - 10.2116/analsci.25.149

DO - 10.2116/analsci.25.149

M3 - Article

C2 - 19212044

AN - SCOPUS:63149149476

SN - 0910-6340

VL - 25

SP - 149

EP - 151

JO - analytical sciences

JF - analytical sciences

IS - 2

ER -

Microchip-based homogeneous immunoassay using a cloned enzyme donor

Abstract

All Science Journal Classification (ASJC) codes

Access to Document

Other files and links

Fingerprint

Cite this