Micro-LED Array-Based Photo-Stimulation Devices for Optogenetics in Rat and Macaque Monkey Brains

Yasumi Ohta, Mark Christian Guinto, Takashi Tokuda, Mamiko Kawahara, Makito Haruta, Hironari Takehara, Hiroyuki Tashiro, Kiyotaka Sasagawa, Hirotaka Onoe, Reona Yamaguchi, Yoshinori Koshimizu, Kaoru Isa, Tadashi Isa, Kenta Kobayashi, Yasemin M. Akay, Metin Akay, Jun Ohta

Research output: Contribution to journalArticlepeer-review

10 Citations (Scopus)


Optogenetics is a powerful tool for controlling biological functions using light. Optical fibers have been extensively utilized in optical stimulation devices for optogenetics. However, the use of optical fibers results in a small photo-stimulation region. In this study, micro-LED array devices were developed to achieve large-area photo-stimulation in the brain of a large animal, such as macaque monkeys. Planar and linear micro-LED array devices were designed and fabricated to photo-stimulate the prefrontal cortex (PFC) and ventral tegmental area (VTA) of the brain and induce a neurochemical response. Device operation, optical intensity, and safety were first characterized using rats. Subsequently, the devices were used to photo-stimulate the brain of macaque monkeys. In addition, microdialysis in the PFC was performed. The devices detected modulated levels of dopamine in the brains. Thus, the photo-stimulation of both the PFC and VTA were successfully achieved, and the effectiveness of the developed micro-LED array devices was demonstrated. The study will help facilitate further studies on micro-LED array stimulation for system-wide optogenetic manipulation in large animals.

Original languageEnglish
Pages (from-to)127937-127949
Number of pages13
JournalIEEE Access
Publication statusPublished - 2021

All Science Journal Classification (ASJC) codes

  • General Computer Science
  • General Materials Science
  • General Engineering


Dive into the research topics of 'Micro-LED Array-Based Photo-Stimulation Devices for Optogenetics in Rat and Macaque Monkey Brains'. Together they form a unique fingerprint.

Cite this