Micro-CT analysis reveals the changes in bone mineral density in zebrafish craniofacial skeleton with age

Wei Neng Liao, May Su You, Zulvikar Syambani Ulhaq, Jui Ping Li, Yun Jin Jiang, Jen Kun Chen, William Ka Fai Tse

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)


Bone has multiple functions in animals, such as supporting the body for mobility. The zebrafish skeleton is composed of craniofacial and axial skeletons. It shares a physiological curvature and consists of a similar number of vertebrae as humans. Bone degeneration and malformations have been widely studied in zebrafish as human disease models. High-resolution imaging and different bone properties such as density and volume can be obtained using micro-computed tomography (micro-CT). This study aimed to understand the possible changes in the structure and bone mineral density (BMD) of the vertebrae and craniofacial skeleton with age (4, 12 and 24 months post fertilisation [mpf]) in zebrafish. Our data showed that the BMD in the vertebrae and specific craniofacial skeleton (mandibular arch, ceratohyal and ethmoid plate) of 12 and 24 mpf fish were higher than that of the 4 mpf fish. In addition, we found the age-dependent increase in BMD was not ubiquitously observed in facial bones, and such differences were not correlated with bone type. In summary, such additional information on the craniofacial skeleton could help in understanding bone development throughout the lifespan of zebrafish.

Original languageEnglish
Pages (from-to)544-551
Number of pages8
JournalJournal of Anatomy
Issue number3
Publication statusPublished - Mar 2023

All Science Journal Classification (ASJC) codes

  • Ecology, Evolution, Behavior and Systematics
  • Molecular Biology
  • Anatomy
  • Cell Biology
  • Histology
  • Developmental Biology


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