TY - JOUR
T1 - Metabolic engineering of oleaginous fungus Mortierella alpina for high production of oleic and linoleic acids
AU - Sakamoto, Takaiku
AU - Sakuradani, Eiji
AU - Okuda, Tomoyo
AU - Kikukawa, Hiroshi
AU - Ando, Akinori
AU - Kishino, Shigenobu
AU - Izumi, Yoshihiro
AU - Bamba, Takeshi
AU - Shima, Jun
AU - Ogawa, Jun
N1 - Funding Information:
This work was supported by a grant of the project of Advanced Low Carbon Technology Research and Development Program (ALCA) of the Japan Science and Technology Agency .
Publisher Copyright:
© 2017 Elsevier Ltd
PY - 2017/12
Y1 - 2017/12
N2 - The aim of this work was to study the molecular breeding of oleaginous filamentous Mortierella alpina for high production of linoleic (LA) or oleic acid (OA). Heterologous expression of the Δ12-desaturase (DS) gene derived from Coprinopsis cinerea in the Δ6DS activity-defective mutant of M. alpina increased the LA production rate as to total fatty acid to 5 times that in the wild strain. By suppressing the endogenous Δ6I gene expression by RNAi in the Δ12DS activity-defective mutant of M. alpina, the OA accumulation rate as to total fatty acid reached 68.0%. The production of LA and OA in these transformants reached 1.44 and 2.76 g/L, respectively, on the 5th day. The Δ6I transcriptional levels of the RNAi-treated strains were suppressed to 1/10th that in the parent strain. The amount of Δ6II RNA in the Δ6I RNAi-treated strain increased to 8 times that in the wild strain.
AB - The aim of this work was to study the molecular breeding of oleaginous filamentous Mortierella alpina for high production of linoleic (LA) or oleic acid (OA). Heterologous expression of the Δ12-desaturase (DS) gene derived from Coprinopsis cinerea in the Δ6DS activity-defective mutant of M. alpina increased the LA production rate as to total fatty acid to 5 times that in the wild strain. By suppressing the endogenous Δ6I gene expression by RNAi in the Δ12DS activity-defective mutant of M. alpina, the OA accumulation rate as to total fatty acid reached 68.0%. The production of LA and OA in these transformants reached 1.44 and 2.76 g/L, respectively, on the 5th day. The Δ6I transcriptional levels of the RNAi-treated strains were suppressed to 1/10th that in the parent strain. The amount of Δ6II RNA in the Δ6I RNAi-treated strain increased to 8 times that in the wild strain.
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U2 - 10.1016/j.biortech.2017.06.089
DO - 10.1016/j.biortech.2017.06.089
M3 - Article
C2 - 28673516
AN - SCOPUS:85021668756
SN - 0960-8524
VL - 245
SP - 1610
EP - 1615
JO - Bioresource Technology
JF - Bioresource Technology
ER -
