Membrane nanotube formation in osteoclastogenesis

Toshio Kukita, Akira Takahashi, Jing Qi Zhang, Akiko Kukita

Research output: Contribution to journalArticlepeer-review

6 Citations (Scopus)


Membrane tunneling nanotubes (TNTs) are unique intercellular structures, which enable rapid transport of various materials and rapid communication between cells present in a long distance. During osteoclas-togenesis, mononuclear osteoclast precursors form abundant TNTs in prior to cell–cell fusion. Here we introduce a protocol for detecting TNTs during osteoclastogenesis by use of live cell imaging utilizing a confocal laser microscopy. We also demonstrate a standard protocol for observation of TNTs by scanning electron microscope.

Original languageEnglish
Pages (from-to)193-202
Number of pages10
JournalMethods in Molecular Biology
Publication statusPublished - 2015

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Genetics


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