Mechanism of hydrolysis of triolein with lipase in a hollow fiber membrane-bioreactor

M. Goto, F. Nakashio, K. Yoshizuka, K. Inoue, Y. Baba

    Research output: Contribution to journalArticlepeer-review

    1 Citation (Scopus)

    Abstract

    The rate of hydrolysis of triolein with lipase in a Teflon hollow fiber membrane bioreactor was quantitatively studied, taking into account the mass transfer of the triolein substrate and the oleic acid reaction product through the membrane. The oleic acid inhibited the hydrolysis at high concentrations, but another reaction product, glycerin, did not inhibit the reaction at all. The hydrolysis of triolein could be explained in terms of a Michaelis-Menten mechanism which takes into account interfacial adsorption and inhibition of the oleic acid. The experimental results could be quantitatively explained by taking simultaneous account of the rate of hydrolysis at the interface and of the rate of mass transfer of triolein and oleic acid to the interface.

    Original languageEnglish
    Pages (from-to)410-416
    Number of pages7
    JournalInternational chemical engineering
    Volume34
    Issue number3
    Publication statusPublished - Jul 1994

    All Science Journal Classification (ASJC) codes

    • General Engineering

    Fingerprint

    Dive into the research topics of 'Mechanism of hydrolysis of triolein with lipase in a hollow fiber membrane-bioreactor'. Together they form a unique fingerprint.

    Cite this