TY - GEN
T1 - Mechanical characterization system of cyanobacteria using a robot integrated microdluidic chip
AU - Hasegawa, Takayuki
AU - Sakuma, Shinya
AU - Nanatani, Kei
AU - Uozumi, Nobuyuki
AU - Arai, Fumihito
N1 - Publisher Copyright:
© 2015 IEEE.
PY - 2015/12/11
Y1 - 2015/12/11
N2 - Cyanobacteria have ion channels which response to osmolarity change in living environment. Since it may lead to the mechanical characteristics changes of the cell itself due to the intracellular pressure changes, we focus on constructing the mechanical characteristics measurement system of Synechocystis sp. PCC 6803, one of unicellular cyanobacteria. There are two important issues in constructing the system. Because of features of the cyanobacteria, which is a kind of floating cell whose diameter is a few micrometer, one is how to transport the cell to the measurement point. The other is how to deform the target cell. In this paper, we propose the system combining optical tweezers for transportation of the target cell with the robot integrated microfluidic chip (robochip). The robochip contains an on-chip probe actuated by an external actuator, and a force sensor. We present the concept of the total system, and demonstrated the results of mechanical characterization of a single cyanobacteria.
AB - Cyanobacteria have ion channels which response to osmolarity change in living environment. Since it may lead to the mechanical characteristics changes of the cell itself due to the intracellular pressure changes, we focus on constructing the mechanical characteristics measurement system of Synechocystis sp. PCC 6803, one of unicellular cyanobacteria. There are two important issues in constructing the system. Because of features of the cyanobacteria, which is a kind of floating cell whose diameter is a few micrometer, one is how to transport the cell to the measurement point. The other is how to deform the target cell. In this paper, we propose the system combining optical tweezers for transportation of the target cell with the robot integrated microfluidic chip (robochip). The robochip contains an on-chip probe actuated by an external actuator, and a force sensor. We present the concept of the total system, and demonstrated the results of mechanical characterization of a single cyanobacteria.
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U2 - 10.1109/IROS.2015.7354236
DO - 10.1109/IROS.2015.7354236
M3 - Conference contribution
AN - SCOPUS:84958174133
T3 - IEEE International Conference on Intelligent Robots and Systems
SP - 6033
EP - 6038
BT - IROS Hamburg 2015 - Conference Digest
PB - Institute of Electrical and Electronics Engineers Inc.
T2 - IEEE/RSJ International Conference on Intelligent Robots and Systems, IROS 2015
Y2 - 28 September 2015 through 2 October 2015
ER -